دورية أكاديمية
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Bioinformatic, Enzymatic, and Structural Characterization of Trichuris suis Hexosaminidase HEX-2.

التفاصيل البيبلوغرافية
العنوان: Bioinformatic, Enzymatic, and Structural Characterization of Trichuris suis Hexosaminidase HEX-2.
المؤلفون: Dutkiewicz Z; Institut für Biochemie, Department für Chemie, Universität für Bodenkultur, Muthgasse 18, Wien 1190, Austria., Varrot A; Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble 38000, France., Breese KJ; School of Molecular Sciences, University of Western Australia, Crawley, WA 6009, Australia., Stubbs KA; School of Molecular Sciences, University of Western Australia, Crawley, WA 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, University of Western Australia, Crawley, WA 6009, Australia., Nuschy L; Institut für Biochemie, Department für Chemie, Universität für Bodenkultur, Muthgasse 18, Wien 1190, Austria., Adduci I; Institut für Parasitologie, Department für Pathobiologie, Veterinärmedizinische Universität Wien, Veterinärplatz 1, Wien A-1210, Austria., Paschinger K; Institut für Biochemie, Department für Chemie, Universität für Bodenkultur, Muthgasse 18, Wien 1190, Austria., Wilson IBH; Institut für Biochemie, Department für Chemie, Universität für Bodenkultur, Muthgasse 18, Wien 1190, Austria.
المصدر: Biochemistry [Biochemistry] 2024 Aug 06; Vol. 63 (15), pp. 1941-1954. Date of Electronic Publication: 2024 Jul 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370623 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4995 (Electronic) Linking ISSN: 00062960 NLM ISO Abbreviation: Biochemistry Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Trichuris*/enzymology , Computational Biology*/methods, Animals ; Substrate Specificity ; Crystallography, X-Ray ; Amino Acid Sequence ; Phylogeny ; Models, Molecular ; Hexosaminidases/chemistry ; Hexosaminidases/metabolism ; Hexosaminidases/genetics ; Molecular Sequence Data ; Catalytic Domain ; Helminth Proteins/chemistry ; Helminth Proteins/metabolism ; Helminth Proteins/genetics ; beta-N-Acetylhexosaminidases/metabolism ; beta-N-Acetylhexosaminidases/chemistry ; beta-N-Acetylhexosaminidases/genetics
مستخلص: Hexosaminidases are key enzymes in glycoconjugate metabolism and occur in all kingdoms of life. Here, we have investigated the phylogeny of the GH20 glycosyl hydrolase family in nematodes and identified a β-hexosaminidase subclade present only in the Dorylaimia. We have expressed one of these, HEX-2 from Trichuris suis , a porcine parasite, and shown that it prefers an aryl β- N -acetylgalactosaminide in vitro . HEX-2 has an almost neutral pH optimum and is best inhibited by GalNAc-isofagomine. Toward N-glycan substrates, it displays a preference for the removal of GalNAc residues from LacdiNAc motifs as well as the GlcNAc attached to the α1,3-linked core mannose. Therefore, it has a broader specificity than insect fused lobe (FDL) hexosaminidases but one narrower than distant homologues from plants. Its X-ray crystal structure, the first of any subfamily 1 GH20 hexosaminidase to be determined, is closest to Streptococcus pneumoniae GH20C and the active site is predicted to be compatible with accommodating both GalNAc and GlcNAc. The new structure extends our knowledge about this large enzyme family, particularly as T. suis HEX-2 also possesses the key glutamate residue found in human hexosaminidases of either GH20 subfamily, including HEXD whose biological function remains elusive.
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المشرفين على المادة: EC 3.2.1.- (Hexosaminidases)
0 (Helminth Proteins)
EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
تواريخ الأحداث: Date Created: 20240726 Date Completed: 20240806 Latest Revision: 20240913
رمز التحديث: 20240913
مُعرف محوري في PubMed: PMC11308363
DOI: 10.1021/acs.biochem.4c00187
PMID: 39058279
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-4995
DOI:10.1021/acs.biochem.4c00187