دورية أكاديمية
In vitro validation of helium ion irradiations as a function of linear energy transfer in radioresistant human malignant cells.
| العنوان: | In vitro validation of helium ion irradiations as a function of linear energy transfer in radioresistant human malignant cells. |
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| المؤلفون: | Fira AMR; Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia., Keta OD; Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia., Petković VD; Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia., Đorđević M; Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia., Petringa G; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy., Fattori S; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy., Catalano R; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy., Cirrone GP; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy., Cuttone G; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy., Sakata D; Division of Health Sciences, Osaka University, Osaka, Japan., Tran NH; University of Bordeaux, CNRS, LP2I, UMR 5797, F-33170 Gradignan, France., Chatzipapas K; University of Brest, Brest, France., Incerti S; University of Bordeaux, CNRS, LP2I, UMR 5797, F-33170 Gradignan, France., Petrović IM; Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia. |
| المصدر: | International journal of radiation biology [Int J Radiat Biol] 2024; Vol. 100 (10), pp. 1426-1437. Date of Electronic Publication: 2024 Jul 26. |
| نوع المنشور: | Journal Article; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 8809243 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-3095 (Electronic) Linking ISSN: 09553002 NLM ISO Abbreviation: Int J Radiat Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; New York : Taylor & Francis, [c1988- |
| مواضيع طبية MeSH: | Helium* , Linear Energy Transfer* , Cell Survival*/radiation effects , Radiation Tolerance*, Humans ; Cell Line, Tumor ; Dose-Response Relationship, Radiation ; DNA Breaks, Double-Stranded/radiation effects ; Histones/metabolism ; Ions |
| مستخلص: | Purpose: Based on considerable interest to enlarge the experimental database of radioresistant cells after their irradiation with helium ions, HTB140, MCF-7 and HTB177 human malignant cells are exposed to helium ion beams having different linear energy transfer (LET). Materials and Methods: The cells are irradiated along the widened 62 MeV/u helium ion Bragg peak, providing LET of 4.9, 9.8, 23.4 and 36.8 keV/µm. Numerical simulations with the Geant4 toolkit are used for the experimental design. Cell survival is evaluated and compared with reference γ-rays. DNA double strand breaks are assessed via γ-H2AX foci. Results: With the increase of LET, surviving fractions at 2 Gy decrease, while RBE (2 Gy, γ) gradually increase. For HTB140 cells, above the dose of 4 Gy, a slight saturation of survival is observed while the increase of RBE (2 Gy, γ) remains unaffected. With the increase of LET the increase of γ-H2AX foci is revealed at 0.5 h after irradiation. There is no significant difference in the number of foci between the cell lines for the same LET. From 0.5 to 24 h, the number of foci drops reaching its residual level. For each time point, there are small differences in DNA DSB among the three cell lines. Conclusion: Analyses of data acquired for the three cell lines irradiated by helium ions, having different LET, reveal high elimination capacity and creation of a large number of DNA DSB with respect to γ-rays, and are between those reported for protons and carbon ions. |
| فهرسة مساهمة: | Keywords: DNA double strand breaks; LET; Modulated helium ions; human malignant radioresistant cells; survival |
| المشرفين على المادة: | 206GF3GB41 (Helium) 0 (H2AX protein, human) 0 (Histones) 0 (Ions) |
| تواريخ الأحداث: | Date Created: 20240726 Date Completed: 20240924 Latest Revision: 20240924 |
| رمز التحديث: | 20240925 |
| DOI: | 10.1080/09553002.2024.2373752 |
| PMID: | 39058324 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1362-3095 |
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| DOI: | 10.1080/09553002.2024.2373752 |