دورية أكاديمية
Exosome Transfer Between Different Co-implanted Human Pancreatic Cancer Cell Lines Occurs in the Primary Tumor and Multiple Metastatic Sites of Nude Mice But Not in Co-Culture In Vitro .
| العنوان: | Exosome Transfer Between Different Co-implanted Human Pancreatic Cancer Cell Lines Occurs in the Primary Tumor and Multiple Metastatic Sites of Nude Mice But Not in Co-Culture In Vitro . |
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| المؤلفون: | Yamashita K; Gifu University Graduate School of Medicine, Gifu, Japan., Suetsugu A; Gifu University Graduate School of Medicine, Gifu, Japan; suetsugu.atsushi.e2@f.gifu-u.ac.jp., Hayashi S; Gifu University Graduate School of Medicine, Gifu, Japan., Shimizu M; Gifu University Graduate School of Medicine, Gifu, Japan., Hoffman RM; AntiCancer, Inc., San Diego, CA, U.S.A.; all@anticancer.com.; Department of Surgery, University of California, San Diego, CA, U.S.A. |
| المصدر: | Anticancer research [Anticancer Res] 2024 Aug; Vol. 44 (8), pp. 3307-3315. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Anticancer Research Country of Publication: Greece NLM ID: 8102988 Publication Model: Print Cited Medium: Internet ISSN: 1791-7530 (Electronic) Linking ISSN: 02507005 NLM ISO Abbreviation: Anticancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Attiki, Greece : International Institute of Anticancer Research Original Publication: Athens, Greece : Potamitis Press |
| مواضيع طبية MeSH: | Exosomes*/metabolism , Pancreatic Neoplasms*/pathology , Pancreatic Neoplasms*/metabolism , Mice, Nude* , Coculture Techniques*, Animals ; Humans ; Cell Line, Tumor ; Mice ; Neoplasm Metastasis ; Luminescent Proteins/metabolism ; Luminescent Proteins/genetics ; Red Fluorescent Protein ; Green Fluorescent Proteins/metabolism ; Green Fluorescent Proteins/genetics |
| مستخلص: | Background/aim: Exosome exchange between cancer cells or between cancer and stromal cells is involved in cancer metastasis. We have previously developed in vivo color-coded labeling of cancer cells and stromal cells with spectrally-distinct fluorescent genetic reporters to demonstrate the role of exosomes in metastasis. In the present study, we studied exosome transfer between different pancreatic-cancer cell lines in vivo and in vitro and its potential role in metastasis. Materials and Methods: Human pancreatic-cancer cell lines AsPC-1 and MiaPaCa-2 were used in the present study. AsPC-1 cells contain a genetic exosome reporter gene labeled with green fluorescent protein (pCT-CD63-GFP) and MiaPaCa-2 cells express red fluorescent protein (RFP). Both cell lines were co-injected into the spleen of nude mice (n=5) to further study the role of exosome exchange in metastasis. Three weeks later mice were sacrificed and tumors at the primary and metastatic sites were cultured and observed by confocal fluorescence microscopy for exosome transfer. Results: The primary tumor formed in the spleen and metastasized to the liver, as observed macroscopically. Cells were cultured from the spleen, liver, lung, bone marrow and ascites. Transfer of exosomes from AsPC-1 to MiaPaCa-2 was demonstrated in the cultured cells by confocal fluorescence microscopy. Moreover, cell fusion was also observed along with exosome transfer. Exosome transfer did not occur during in vitro co-culture between the two pancreatic-cancer cell lines, suggesting a role of the tumor microenvironment (TME) in exosome transfer. Conclusion: The transfer of exosomes between different pancreatic-cancer cell lines was observed during primary-tumor and metastatic growth in nude mice. This cell-cell communication might be a trigger of cell fusion and promotion of cancer metastasis. Exosome transfer between the two pancreatic-cancer cell lines appears to be facilitated by the TME, as it did not occur during in vitro co-culture. (Copyright © 2024 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.) |
| فهرسة مساهمة: | Keywords: AsPC-1; GFP; MiaPaCa-2; RFP; cell fusion; color-coded imaging; exosome; metastasis; nude mice; transfer |
| المشرفين على المادة: | 0 (Luminescent Proteins) 0 (Red Fluorescent Protein) 147336-22-9 (Green Fluorescent Proteins) |
| تواريخ الأحداث: | Date Created: 20240726 Date Completed: 20240728 Latest Revision: 20240728 |
| رمز التحديث: | 20240728 |
| DOI: | 10.21873/anticanres.17149 |
| PMID: | 39060068 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1791-7530 |
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| DOI: | 10.21873/anticanres.17149 |