دورية أكاديمية
In silico designing of multi-epitope vaccine against canine parvovirus using reverse vaccinology.
| العنوان: | In silico designing of multi-epitope vaccine against canine parvovirus using reverse vaccinology. |
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| المؤلفون: | Lopes TS; Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil., Gheno BP; Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil., Miranda LDS; Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil., Detofano J; Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil., Khan MAA; University of Leipzig, Leipzig, Germany., Streck AF; Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil. afstreck@ucs.br. |
| المصدر: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Sep; Vol. 55 (3), pp. 2953-2968. Date of Electronic Publication: 2024 Jul 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Switzerland, AG : Springer International Publishing Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia |
| مواضيع طبية MeSH: | Parvovirus, Canine*/immunology , Parvovirus, Canine*/genetics , Parvovirus, Canine*/chemistry , Dog Diseases*/prevention & control , Dog Diseases*/immunology , Dog Diseases*/virology , Parvoviridae Infections*/prevention & control , Parvoviridae Infections*/veterinary , Parvoviridae Infections*/immunology , Viral Vaccines*/immunology , Viral Vaccines*/genetics , Viral Vaccines*/chemistry , Computer Simulation*, Animals ; Dogs ; Brazil ; Vaccinology/methods ; Capsid Proteins/immunology ; Capsid Proteins/genetics ; Capsid Proteins/chemistry ; Epitopes, B-Lymphocyte/immunology ; Epitopes, B-Lymphocyte/genetics ; Epitopes/immunology ; Epitopes/genetics ; Epitopes/chemistry ; Epitopes, T-Lymphocyte/immunology ; Epitopes, T-Lymphocyte/genetics ; Epitopes, T-Lymphocyte/chemistry |
| مستخلص: | Canine parvovirus (CPV-2) is a highly contagious virus affecting dogs worldwide, posing a significant threat. The VP2 protein stands out as the predominant and highly immunogenic structural component of CPV-2. Soon after its emergence, CPV-2 was replaced by variants known as CPV-2a, 2b and 2c, marked by changes in amino acid residue 426 of VP2. Additional amino acid alterations have been identified within VP2, with certain modifications serving as signatures of emerging variants. In Brazil, CPV-2 outbreaks persist with diverse VP2 profiles. Vaccination is the main preventive measure against the virus. However, the emergence of substitutions presents challenges to conventional vaccine methods. Commercial vaccines are formulated with strains that usually do not match those currently circulating in the field. To address this, the study aimed to investigate CPV-2 variants in Brazil, predict epitopes, and design an in silico vaccine tailored to local variants employing reverse vaccinology. The methodology involved data collection, genetic sequence analysis, and amino acid comparison between field strains and vaccines, followed by the prediction of B and T cell epitope regions. The predicted epitopes were evaluated for antigenicity, allergenicity and toxicity. The final vaccine construct consisted of selected epitopes linked to an adjuvant and optimized for expression in Escherichia coli. Structural predictions confirmed the stability and antigenicity of the vaccine, while molecular docking demonstrated interaction with the canine toll-like receptor 4. Molecular dynamics simulations indicated a stable complex formation. In silico immune simulations demonstrated a progressive immune response post-vaccination, including increased antibody production and T-helper cell activity. The multi-epitope vaccine design targeted prevalent CPV-2 variants in Brazil and potentially other regions globally. However, experimental validation is essential to confirm our in silico findings. (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.) |
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| معلومات مُعتمدة: | Conselho Nacional de Desenvolvimento Científico e Tecnológico Conselho Nacional de Desenvolvimento Científico e Tecnológico; 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul |
| فهرسة مساهمة: | Keywords: Brazil; Canine parvovirus; Epitope; Immunoinformatics; Vaccine |
| المشرفين على المادة: | 0 (Viral Vaccines) 0 (Capsid Proteins) 0 (Epitopes, B-Lymphocyte) 0 (Epitopes) 0 (Epitopes, T-Lymphocyte) |
| تواريخ الأحداث: | Date Created: 20240726 Date Completed: 20240916 Latest Revision: 20240919 |
| رمز التحديث: | 20240919 |
| مُعرف محوري في PubMed: | PMC11405728 |
| DOI: | 10.1007/s42770-024-01442-7 |
| PMID: | 39060911 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1678-4405 |
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| DOI: | 10.1007/s42770-024-01442-7 |