دورية أكاديمية
Promoter of Cassava MeAHL31 Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic Arabidopsis .
| العنوان: | Promoter of Cassava MeAHL31 Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic Arabidopsis . |
|---|---|
| المؤلفون: | Wang XT; National Key Laboratory for Tropical Crop Breeding, School of Life and Health Sciences, Hainan University, Haikou 570228, China., Tang XN; National Key Laboratory for Tropical Crop Breeding, School of Life and Health Sciences, Hainan University, Haikou 570228, China., Zhang YW; National Key Laboratory for Tropical Crop Breeding, School of Life and Health Sciences, Hainan University, Haikou 570228, China., Guo YQ; National Key Laboratory for Tropical Crop Breeding, School of Life and Health Sciences, Hainan University, Haikou 570228, China., Yao Y; National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China., Li RM; National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China., Wang YJ; National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China., Liu J; National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China., Guo JC; National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2024 Jul 14; Vol. 25 (14). Date of Electronic Publication: 2024 Jul 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Arabidopsis*/genetics , Arabidopsis*/metabolism , Plants, Genetically Modified*/genetics , Promoter Regions, Genetic* , Gene Expression Regulation, Plant* , Stress, Physiological*/genetics , Manihot*/genetics , Manihot*/metabolism , Plant Proteins*/genetics , Plant Proteins*/metabolism , Plant Growth Regulators*/metabolism , Plant Growth Regulators*/pharmacology, Droughts ; Cyclopentanes/pharmacology ; Cyclopentanes/metabolism ; Abscisic Acid/pharmacology ; Abscisic Acid/metabolism ; Oxylipins/pharmacology ; Oxylipins/metabolism ; Acetates/pharmacology |
| مستخلص: | The AT-hook motif nuclear-localized ( AHL ) family is pivotal for the abiotic stress response in plants. However, the function of the cassava AHL genes has not been elucidated. Promoters, as important regulatory elements of gene expression, play a crucial role in stress resistance. In this study, the promoter of the cassava MeAHL31 gene was cloned. The MeAHL31 protein was localized to the cytoplasm and the nucleus. qRT-PCR analysis revealed that the MeAHL31 gene was expressed in almost all tissues tested, and the expression in tuber roots was 321.3 times higher than that in petioles. Promoter analysis showed that the MeAHL31 promoter contains drought, methyl jasmonate (MeJA), abscisic acid (ABA), and gibberellin (GA) cis -acting elements. Expression analysis indicated that the MeAHL31 gene is dramatically affected by treatments with salt, drought, MeJA, ABA, and GA3. Histochemical staining in the proMeAHL31-GUS transgenic Arabidopsis corroborated that the GUS staining was found in most tissues and organs, excluding seeds. Beta-glucuronidase (GUS) activity assays showed that the activities in the proMeAHL31-GUS transgenic Arabidopsis were enhanced by different concentrations of NaCl, mannitol (for simulating drought), and MeJA treatments. The integrated findings suggest that the MeAHL31 promoter responds to the abiotic stresses of salt and drought, and its activity is regulated by the MeJA hormone signal. |
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| فهرسة مساهمة: | Keywords: AT-hook protein; GUS; MeAHL31; abiotic stresses; cassava; hormone signals; promoter; transgenic Arabidopsis |
| المشرفين على المادة: | 0 (Plant Proteins) 0 (Plant Growth Regulators) 0 (Cyclopentanes) 72S9A8J5GW (Abscisic Acid) 0 (Oxylipins) 0 (Acetates) 900N171A0F (methyl jasmonate) |
| تواريخ الأحداث: | Date Created: 20240727 Date Completed: 20240727 Latest Revision: 20240729 |
| رمز التحديث: | 20240729 |
| مُعرف محوري في PubMed: | PMC11276720 |
| DOI: | 10.3390/ijms25147714 |
| PMID: | 39062957 |
| قاعدة البيانات: | MEDLINE |
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