دورية أكاديمية
أعرض في EDS

Sulforaphane Effects on Neuronal-like Cells and Peripheral Blood Mononuclear Cells Exposed to 2.45 GHz Electromagnetic Radiation.

التفاصيل البيبلوغرافية
العنوان: Sulforaphane Effects on Neuronal-like Cells and Peripheral Blood Mononuclear Cells Exposed to 2.45 GHz Electromagnetic Radiation.
المؤلفون: Bertuccio MP; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy., Saija C; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy., Acri G; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy., Ientile R; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy., Caccamo D; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy., Currò M; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Jul 18; Vol. 25 (14). Date of Electronic Publication: 2024 Jul 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Isothiocyanates*/pharmacology , Sulfoxides*/pharmacology , Leukocytes, Mononuclear*/radiation effects , Leukocytes, Mononuclear*/drug effects , Leukocytes, Mononuclear*/metabolism , Neurons*/radiation effects , Neurons*/drug effects , Neurons*/metabolism , Oxidative Stress*/drug effects , Oxidative Stress*/radiation effects , Membrane Potential, Mitochondrial*/drug effects , Membrane Potential, Mitochondrial*/radiation effects , Electromagnetic Radiation*, Humans ; Cell Survival/drug effects ; Cell Survival/radiation effects ; Apoptosis/drug effects ; Apoptosis/radiation effects ; Reactive Oxygen Species/metabolism ; NF-E2-Related Factor 2/metabolism ; NF-E2-Related Factor 2/genetics ; Mitochondria/drug effects ; Mitochondria/radiation effects ; Mitochondria/metabolism ; Cell Line, Tumor
مستخلص: Exposure to 2.45 GHz electromagnetic radiation (EMR) emitted from commonly used devices has been reported to induce oxidative stress in several experimental models. Our study aims to evaluate the efficacy of sulforaphane, a well-known natural product, in preventing radiation-induced toxic effects caused by a 24 h exposure of SH-SY5Y neuronal-like cells and peripheral blood mononuclear cells (PBMCs) to 2.45 GHz EMR. Cells were exposed to radiation for 24 h in the presence or absence of sulforaphane at different concentrations (5-10-25 µg/mL). Cell viability, mitochondrial activity alterations, the transcription and protein levels of redox markers, and apoptosis-related genes were investigated. Our data showed a reduction in cell viability of both neuronal-like cells and PBMCs caused by EMR exposure and a protective effect of 5 µg/mL sulforaphane. The lowest sulforaphane concentration decreased ROS production and increased the Mitochondrial Transmembrane Potential (Δ ψ m ) and the NAD+/NADH ratio, which were altered by radiation exposure. Sulforaphane at higher concentrations displayed harmful effects. The hormetic behavior of sulforaphane was also evident after evaluating the expression of genes coding for Nrf2, SOD2, and changes in apoptosis markers. Our study underlined the vulnerability of neuronal-like cells to mitochondrial dysfunction and oxidative stress and the possibility of mitigating these effects by supplementation with sulforaphane. To our knowledge, there are no previous studies about the effects of SFN on these cells when exposed to 2.45 GHz electromagnetic radiation.
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فهرسة مساهمة: Keywords: electromagnetic radiation; hormetic effects; neuronal-like cells; peripheral blood mononuclear cells; sulforaphane
المشرفين على المادة: GA49J4310U (sulforaphane)
0 (Isothiocyanates)
0 (Sulfoxides)
0 (Reactive Oxygen Species)
0 (NF-E2-Related Factor 2)
تواريخ الأحداث: Date Created: 20240727 Date Completed: 20240727 Latest Revision: 20240729
رمز التحديث: 20240729
مُعرف محوري في PubMed: PMC11276899
DOI: 10.3390/ijms25147872
PMID: 39063113
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25147872