دورية أكاديمية
Assessing the mechanism of facilitated proton transport across GUVs trapped in a microfluidic device.
| العنوان: | Assessing the mechanism of facilitated proton transport across GUVs trapped in a microfluidic device. |
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| المؤلفون: | Ruppelt D; Institute of Organic and Biomolecular Chemistry, Georg-August Universität Göttingen, Göttingen, Germany., Ackermann ELM; Institute of Organic and Biomolecular Chemistry, Georg-August Universität Göttingen, Göttingen, Germany., Robinson T; Institute for Bioengineering, School of Engineering, University of Edinburgh, Edinburgh, United Kingdom., Steinem C; Institute of Organic and Biomolecular Chemistry, Georg-August Universität Göttingen, Göttingen, Germany. Electronic address: csteine@gwdg.de. |
| المصدر: | Biophysical journal [Biophys J] 2024 Sep 17; Vol. 123 (18), pp. 3267-3274. Date of Electronic Publication: 2024 Jul 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-0086 (Electronic) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society. |
| مواضيع طبية MeSH: | Protons* , Unilamellar Liposomes*/chemistry , Unilamellar Liposomes*/metabolism , Ion Transport* , Lab-On-A-Chip Devices*, Hydrogen-Ion Concentration ; Gramicidin/metabolism ; Gramicidin/chemistry ; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology ; Arylsulfonates/chemistry ; Arylsulfonates/metabolism |
| مستخلص: | Proton transport across lipid membranes is one of the most fundamental reactions that make up living organisms. In vitro, however, the study of proton transport reactions can be very challenging due to limitations imposed by proton concentrations, compartment size, and unstirred layers as well as buffer exchange and buffer capacity. In this study, we have developed a proton permeation assay based on the microfluidic trapping of giant vesicles enclosing the pH-sensitive dye pyranine to address some of these challenges. Time-resolved fluorescence imaging upon a rapid pH shift enabled us to investigate the facilitated H + permeation mediated by either a channel or a carrier. Specifically, we compared the proton transport rates as a function of different proton gradients of the channel gramicidin D and the proton carrier carbonyl cyanide-m-chlorophenyl hydrazone. Our results demonstrate the efficacy of the assay in monitoring proton transport reactions and distinguishing between a channel-like and a carrier-like mechanism. This groundbreaking result enabled us to elucidate the enigmatic mode of the proton permeation mechanism of the recently discovered natural fibupeptide lugdunin. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Protons) 0 (Unilamellar Liposomes) I2W85YOX9L (pyranine) 1405-97-6 (Gramicidin) 555-60-2 (Carbonyl Cyanide m-Chlorophenyl Hydrazone) 0 (Arylsulfonates) |
| تواريخ الأحداث: | Date Created: 20240727 Date Completed: 20240918 Latest Revision: 20240920 |
| رمز التحديث: | 20240920 |
| DOI: | 10.1016/j.bpj.2024.07.030 |
| PMID: | 39066477 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1542-0086 |
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| DOI: | 10.1016/j.bpj.2024.07.030 |