دورية أكاديمية
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Improving Sensitivity and Longevity of In Vivo Glutamate Sensors with Electrodeposited NanoPt.

التفاصيل البيبلوغرافية
العنوان: Improving Sensitivity and Longevity of In Vivo Glutamate Sensors with Electrodeposited NanoPt.
المؤلفون: Robbins EM; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States., Wong B; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.; Department of Anesthesiology & Perioperative Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, United States., Pwint MY; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.; Center for Neural Basis of Cognition, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States., Salavatian S; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.; Department of Anesthesiology & Perioperative Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, United States., Mahajan A; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.; Department of Anesthesiology & Perioperative Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, United States., Cui XT; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.; Center for Neural Basis of Cognition, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.
المصدر: ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2024 Aug 07; Vol. 16 (31), pp. 40570-40580. Date of Electronic Publication: 2024 Jul 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Chemical Society
مواضيع طبية MeSH: Biosensing Techniques*/instrumentation , Biosensing Techniques*/methods , Glutamic Acid*/analysis , Glutamic Acid*/chemistry , Amino Acid Oxidoreductases*/chemistry , Amino Acid Oxidoreductases*/metabolism, Animals ; Rats ; Electrodes ; Platinum/chemistry ; Swine ; Brain Injuries, Traumatic/metabolism ; Electrochemical Techniques/methods ; Electrochemical Techniques/instrumentation ; Hydrogen Peroxide/analysis ; Hydrogen Peroxide/metabolism ; Hydrogen Peroxide/chemistry ; Rats, Sprague-Dawley ; Male ; Electroplating
مستخلص: In vivo glutamate sensing has provided valuable insight into the physiology and pathology of the brain. Electrochemical glutamate biosensors, constructed by cross-linking glutamate oxidase onto an electrode and oxidizing H 2 O 2 as a proxy for glutamate, are the gold standard for in vivo glutamate measurements for many applications. While glutamate sensors have been employed ubiquitously for acute measurements, there are almost no reports of long-term, chronic glutamate sensing in vivo, despite demonstrations of glutamate sensors lasting for weeks in vitro. To address this, we utilized a platinum electrode with nanometer-scale roughness (nanoPt) to improve the glutamate sensors' sensitivity and longevity. NanoPt improved the GLU sensitivity by 67.4% and the sensors were stable in vitro for 3 weeks. In vivo, nanoPt glutamate sensors had a measurable signal above a control electrode on the same array for 7 days. We demonstrate the utility of the nanoPt sensors by studying the effect of traumatic brain injury on glutamate in the rat striatum with a flexible electrode array and report measurements of glutamate taken during the injury itself. We also show the flexibility of the nanoPt platform to be applied to other oxidase enzyme-based biosensors by measuring γ-aminobutyric acid in the porcine spinal cord. NanoPt is a simple, effective way to build high sensitivity, robust biosensors harnessing enzymes to detect neurotransmitters in vivo.
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معلومات مُعتمدة: R01 NS110564 United States NS NINDS NIH HHS; R21 NS123937 United States NS NINDS NIH HHS; R21 NS125461 United States NS NINDS NIH HHS
فهرسة مساهمة: Keywords: GABA sensor; enzymatic sensor stability; glutamate sensor; in vivo sensing; nanoplatinum; surface modification
المشرفين على المادة: 3KX376GY7L (Glutamic Acid)
EC 1.4.- (Amino Acid Oxidoreductases)
49DFR088MY (Platinum)
BBX060AN9V (Hydrogen Peroxide)
تواريخ الأحداث: Date Created: 20240730 Date Completed: 20240808 Latest Revision: 20240811
رمز التحديث: 20240812
مُعرف محوري في PubMed: PMC11310907
DOI: 10.1021/acsami.4c06692
PMID: 39078097
قاعدة البيانات: MEDLINE
الوصف
تدمد:1944-8252
DOI:10.1021/acsami.4c06692