دورية أكاديمية
Functional OmpA of Salmonella Typhimurium provides protection from lysosomal degradation and inhibits autophagic processes in macrophages.
| العنوان: | Functional OmpA of Salmonella Typhimurium provides protection from lysosomal degradation and inhibits autophagic processes in macrophages. |
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| المؤلفون: | Roy Chowdhury A; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India-560012., Hajra D; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India-560012., Mukherjee D; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India-560012., Nair AV; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India-560012., Chakravortty D; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India-560012.; School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695551, India. |
| المصدر: | The Journal of infectious diseases [J Infect Dis] 2024 Jul 30. Date of Electronic Publication: 2024 Jul 30. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1537-6613 (Electronic) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press |
| مستخلص: | Our previous study showed that OmpA-deficient Salmonella Typhimurium (STM) failed to retain LAMP-1, quit Salmonella-containing vacuole (SCV) and escaped to the host cytosol. Here we show that the cytosolic population of STM ΔompA sequestered autophagic markers, syntaxin17 and LC3B in a sseL-dependent manner and initiated lysosomal fusion. Moreover, inhibition of autophagy using bafilomycinA1 restored its intracellular proliferation. Ectopic overexpression of OmpA in STM ΔsifA restored its vacuolar niche and increased interaction of LAMP-1, suggesting a sifA-independent role of OmpA in maintaining an intact SCV. The OmpA extracellular loops impaired the LAMP-1 recruitment to SCV and caused bacterial release into the cytosol of macrophages, but unlike STM ΔompA, they retained their outer membrane stability and didn't activate the lysosomal degradation pathway aiding in their intra-macrophage survival. Finally, OmpA extracellular loop mutations protected the cytosolic STM ΔsifA from the lysosomal surveillance, revealing a unique OmpA-dependent strategy of STM for its intracellular survival. (© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: Salmonella-containing vacuole (SCV); EEA-1; LAMP-1; LC3B; OmpA extracellular loops; lysosomes; outer membrane protein A (OmpA); syntaxin 17 |
| تواريخ الأحداث: | Date Created: 20240730 Latest Revision: 20240730 |
| رمز التحديث: | 20240731 |
| DOI: | 10.1093/infdis/jiae376 |
| PMID: | 39078938 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1537-6613 |
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| DOI: | 10.1093/infdis/jiae376 |