دورية أكاديمية
Coordination of oxysterol binding protein 1 and VAP-A/B modulates the generation of cholesterol and viral inclusion bodies to promote grass carp reovirus replication.
| العنوان: | Coordination of oxysterol binding protein 1 and VAP-A/B modulates the generation of cholesterol and viral inclusion bodies to promote grass carp reovirus replication. |
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| المؤلفون: | Li JQ; Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, College of Life Sciences, Chongqing Normal University, Chongqing, China.; Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China., Zhang J; Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.; University of Chinese Academy of Sciences, Beijing, China., Chen Y; Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.; University of Chinese Academy of Sciences, Beijing, China., Le T; Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, College of Life Sciences, Chongqing Normal University, Chongqing, China., Chang MX; Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.; University of Chinese Academy of Sciences, Beijing, China. |
| المصدر: | Frontiers in immunology [Front Immunol] 2024 Jul 16; Vol. 15, pp. 1419321. Date of Electronic Publication: 2024 Jul 16 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Virus Replication* , Reoviridae*/physiology , Carps*/virology , Carps*/metabolism , Inclusion Bodies, Viral*/metabolism , Cholesterol*/metabolism , Receptors, Steroid*/metabolism, Animals ; Fish Diseases/virology ; Fish Diseases/metabolism ; Fish Diseases/immunology ; Host-Pathogen Interactions ; Reoviridae Infections/veterinary ; Reoviridae Infections/metabolism ; Reoviridae Infections/virology ; Fish Proteins/metabolism ; Fish Proteins/genetics ; Viral Nonstructural Proteins/metabolism ; Viral Nonstructural Proteins/genetics |
| مستخلص: | Similar to other RNA viruses, grass carp reovirus, the causative agent of the hemorrhagic disease, replicates in cytoplasmic viral inclusion bodies (VIBs), orchestrated by host proteins and lipids. The host pathways that facilitate the formation and function of GCRV VIBs are poorly understood. This work demonstrates that GCRV manipulates grass carp oxysterol binding protein 1 (named as gcOSBP1) and vesicle-associated membrane protein-associated protein A/B (named as gcVAP-A/B), 3 components of cholesterol transport pathway, to generate VIBs. By siRNA-mediated knockdown, we demonstrate that gcOSBP1 is an essential host factor for GCRV replication. We reveal that the nonstructural proteins NS80 and NS38 of GCRV interact with gcOSBP1, and that the gcOSBP1 is recruited by NS38 and NS80 for promoting the generation of VIBs. gcOSBP1 increases the expression of gcVAP-A/B and promotes the accumulation of intracellular cholesterol. gcOSBP1 also interacts with gcVAP-A/B for forming gcOSBP1-gcVAP-A/B complexes, which contribute to enhance the accumulation of intracellular cholesterol and gcOSBP1-mediated generation of VIBs. Inhibiting cholesterol accumulation by lovastatin can completely abolish the effects of gcOSBP1 and/or gcVAP-A/B in promoting GCRV infection, suggesting that cholesterol accumulation is vital for gcOSBP1- and/or gcVAP-A/B-mediated GCRV replication. Thus, our results, which highlight that gcOSBP1 functions in the replication of GCRV via its interaction with essential viral proteins for forming VIBs and with host gcVAP-A/B, provide key molecular targets for obtaining anti-hemorrhagic disease grass carp via gene editing technology. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Li, Zhang, Chen, Le and Chang.) |
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| فهرسة مساهمة: | Keywords: VAP-A/B; cholesterol; grass carp OSBP1; grass carp reovirus; viral inclusion bodies |
| المشرفين على المادة: | 0 (oxysterol binding protein) 97C5T2UQ7J (Cholesterol) 0 (Receptors, Steroid) 0 (Fish Proteins) 0 (Viral Nonstructural Proteins) |
| تواريخ الأحداث: | Date Created: 20240731 Date Completed: 20240731 Latest Revision: 20240801 |
| رمز التحديث: | 20240801 |
| مُعرف محوري في PubMed: | PMC11286474 |
| DOI: | 10.3389/fimmu.2024.1419321 |
| PMID: | 39081319 |
| قاعدة البيانات: | MEDLINE |
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