دورية أكاديمية
أعرض في EDS

Hydroxy Fatty Acid Synthesis-Related mRNA as the Biomarker for Detecting Mislabeling of Honey Entomological Origin.

التفاصيل البيبلوغرافية
العنوان: Hydroxy Fatty Acid Synthesis-Related mRNA as the Biomarker for Detecting Mislabeling of Honey Entomological Origin.
المؤلفون: Wang Y; College of Food Science and Technology, Northwest University, 229 North Taibai Road, Xi'an 710069, China., Wu J; College of Food Science and Technology, Northwest University, 229 North Taibai Road, Xi'an 710069, China., Wang G; College of Food Science and Technology, Northwest University, 229 North Taibai Road, Xi'an 710069, China., Tang W; College of Food Science and Technology, Northwest University, 229 North Taibai Road, Xi'an 710069, China., Wu F; College of Food Science and Technology, Northwest University, 229 North Taibai Road, Xi'an 710069, China., Zhao H; College of Food Science and Technology, Northwest University, 229 North Taibai Road, Xi'an 710069, China.; Bee Product Research Center of Shaanxi Province, Xi'an 710065, China., Cao W; College of Food Science and Technology, Northwest University, 229 North Taibai Road, Xi'an 710069, China.; Bee Product Research Center of Shaanxi Province, Xi'an 710065, China.
المصدر: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Aug 14; Vol. 72 (32), pp. 18283-18293. Date of Electronic Publication: 2024 Jul 31.
نوع المنشور: Journal Article; Evaluation Study
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Honey*/analysis , Fatty Acids*/metabolism , Fatty Acids*/analysis , RNA, Messenger*/genetics , RNA, Messenger*/analysis , Insect Proteins*/genetics , Insect Proteins*/metabolism , Biomarkers*/analysis, Animals ; Bees/genetics ; Bees/metabolism ; Food Contamination/analysis
مستخلص: The authentication of the entomological origin of honey is a widespread concern, necessitating the prompt establishment of an effective approach for distinguishing between Apis cerana cerana honey (ACH) and Apis mellifera ligustica honey (AMH). Hydroxy fatty acids (HFAs) found in honey are bee-derived components synthesized by the mandibular glands of worker bees. We previously discovered significant variations in the hydroxy fatty acid composition between ACH and AMH, suggesting their potential as indicators for identifying the authenticity of the entomological origin of honey. Herein, we identified differentially expressed genes associated with HFA synthesis by conducting transcriptome sequencing of the mandibular glands of AC and AM honeybees. Subsequently, we proposed a method for the relative quantitative analysis of bee-derived RNA components using real-time fluorescence quantitative polymerase chain reaction, which was supplemented by multivariate statistical analysis to further discern differences in HFA synthesis-related mRNA between ACH and AMH. The results showed that the mRNAs of FAXDC2 (fatty acid hydroxylase domain-containing protein 2) and FAS (fatty acid synthase) may serve as indicators to discern the entomological origin of honey. This study presents two novel biomarkers for detecting mislabeling of the entomological origin in ACH and AMH based on variations in bee-derived components.
فهرسة مساهمة: Keywords: RT-qPCR; authenticity; entomological origin; honey; transcriptomics
المشرفين على المادة: 0 (Fatty Acids)
0 (RNA, Messenger)
0 (Insect Proteins)
0 (Biomarkers)
تواريخ الأحداث: Date Created: 20240731 Date Completed: 20240814 Latest Revision: 20240814
رمز التحديث: 20240814
DOI: 10.1021/acs.jafc.4c03934
PMID: 39082820
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5118
DOI:10.1021/acs.jafc.4c03934