دورية أكاديمية
Comparative proteomic analysis of PK-15 cells infected with wild-type strain and its EP0 gene-deleted mutant strain of pseudorabies virus.
| العنوان: | Comparative proteomic analysis of PK-15 cells infected with wild-type strain and its EP0 gene-deleted mutant strain of pseudorabies virus. |
|---|---|
| المؤلفون: | Wang D; School of Agroforestry and Medicine, The Open University of China, Beijing 100039, China., Chen D; Institute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China. chendongjie1212@163.com., Xu S; College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China., Wei F; Institute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China., Zhao H; School of Modern Agriculture & Biotechnology, Ankang University, Ankang 725000, China. zhaohongyuan18@163.com. |
| المصدر: | Journal of veterinary science [J Vet Sci] 2024 Jul; Vol. 25 (4), pp. e54. |
| نوع المنشور: | Journal Article; Comparative Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Society of Veterinary Science Country of Publication: Korea (South) NLM ID: 100964185 Publication Model: Print Cited Medium: Internet ISSN: 1976-555X (Electronic) Linking ISSN: 1229845X NLM ISO Abbreviation: J Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Seoul : Korean Society of Veterinary Science, 2000- |
| مواضيع طبية MeSH: | Herpesvirus 1, Suid*/physiology , Herpesvirus 1, Suid*/genetics , Proteomics*, Animals ; Swine ; Cell Line ; Gene Deletion ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Pseudorabies/virology ; Pseudorabies/genetics ; Proteome ; Swine Diseases/virology ; Swine Diseases/genetics ; Swine Diseases/metabolism |
| مستخلص: | Importance: As one of the main etiologic agents of infectious diseases in pigs, pseudorabies virus (PRV) infections have caused enormous economic losses worldwide. EP0, one of the PRV early proteins (EP) plays a vital role in PRV infections, but the mechanisms are unclear. Objective: This study examined the function of EP0 to provide a direction for its in-depth analysis. Methods: In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells. Results: This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization. Conclusions and Relevance: These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections. Competing Interests: The authors declare no conflicts of interest. (© 2024 The Korean Society of Veterinary Science.) |
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| معلومات مُعتمدة: | 2023YFF0614402 China China National Key R&D Program; 31902302 China NSFC National Natural Science Foundation of China |
| فهرسة مساهمة: | Keywords: Pseudorabies virus; Sp100; gene ontology; proteomics; reverse genetics |
| المشرفين على المادة: | 0 (Viral Proteins) 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20240731 Date Completed: 20240731 Latest Revision: 20240803 |
| رمز التحديث: | 20240803 |
| مُعرف محوري في PubMed: | PMC11291433 |
| DOI: | 10.4142/jvs.24069 |
| PMID: | 39083206 |
| قاعدة البيانات: | MEDLINE |
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