دورية أكاديمية
أعرض في EDS

Utility of pan-bacterial and pan-fungal PCR in endophthalmitis: case report and review of the literature.

التفاصيل البيبلوغرافية
العنوان: Utility of pan-bacterial and pan-fungal PCR in endophthalmitis: case report and review of the literature.
المؤلفون: Ercanbrack CW; College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Rahal DA; College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Chauhan MZ; Harvey and Bernice Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Jabbehdari S; Harvey and Bernice Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Uwaydat SH; Harvey and Bernice Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, AR, USA. SHUwaydat@uams.edu.; Department of Ophthalmology, Jones Eye Institute, University of Arkansas for Medical Sciences, 4301 W Markham Street # 523, Little Rock, AR, 72205, USA. SHUwaydat@uams.edu.
المصدر: Journal of ophthalmic inflammation and infection [J Ophthalmic Inflamm Infect] 2024 Aug 01; Vol. 14 (1), pp. 37. Date of Electronic Publication: 2024 Aug 01.
نوع المنشور: Journal Article; Review
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Germany NLM ID: 101553216 Publication Model: Electronic Cited Medium: Print ISSN: 1869-5760 (Print) Linking ISSN: 18695760 NLM ISO Abbreviation: J Ophthalmic Inflamm Infect Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Springer
مستخلص: Background: Endophthalmitis is a clinical diagnosis but identification of the disease-causing agent or agents allows for a more tailored treatment. This is routinely done through intraocular fluid cultures and staining. However, culture-negative endophthalmitis is a relatively common occurrence, and a causative organism cannot be identified. Thus, further diagnostic testing, such as pan-bacterial and pan-fungal polymerase chain reactions (PCRs), may be required. BODY: There are now newer, other testing modalities, specifically pan-bacterial and pan-fungal PCRs, that may allow ophthalmologists to isolate a causative agent when quantitative PCRs and cultures remain negative. We present a case report in which pan-fungal PCR was the only test, amongst quantitative PCRs, cultures, and biopsies, that was able to identify a pathogen in endophthalmitis. Pan-PCR has unique advantages over quantitative PCR in that it does not have a propensity for false-positive results due to contamination. Conversely, pan-PCR has drawbacks, including its inability to detect viruses and parasites and its increased turnaround time and cost. Based on two large retrospective studies, pan-PCR was determined not to be recommended in routine cases of systemic infection as it does not typically add value to the diagnostic workup and does not change the treatment course in most cases. However, in cases like the one presented, pan-bacterial and pan-fungal PCRs may be considered if empiric treatment fails or if the infective organism cannot be isolated. If pan-PCR remains negative or endophthalmitis continues to persist, an even newer form of testing, next-generation sequencing, may aid in the diagnostic workup of culture-negative endophthalmitis.
Conclusion: Pan-bacterial and pan-fungal PCR testing is a relatively new diagnostic tool with unique advantages and drawbacks compared to traditional culturing and PCR methods. Similar to the tests' use in non-ophthalmic systemic infections, pan-bacterial and pan-fungal PCRs are unlikely to become the initial diagnosis test and completely replace culture methods. However, they can provide useful diagnostic information if an infectious agent is unable to be identified with traditional methods or if empiric treatment of endophthalmitis continues to fail.
(© 2024. The Author(s).)
References: J Mol Diagn. 2015 Nov;17(6):623-34. (PMID: 26433313)
JCO Precis Oncol. 2020 Sep 21;4:. (PMID: 33015530)
Semin Ophthalmol. 2023 Jan;38(1):92-98. (PMID: 35982639)
Invest Ophthalmol Vis Sci. 2011 Feb 22;52(2):873-81. (PMID: 20702819)
J Family Med Prim Care. 2021 Jul;10(7):2533-2540. (PMID: 34568132)
J Ophthalmol. 2022 Aug 27;2022:4024260. (PMID: 36065285)
Front Cell Infect Microbiol. 2022 Sep 14;12:899508. (PMID: 36189371)
Am J Ophthalmol. 2020 Apr;212:34-42. (PMID: 31770517)
Front Cell Infect Microbiol. 2020 Sep 23;10:528884. (PMID: 33072623)
Sci Rep. 2020 May 14;10(1):7965. (PMID: 32409679)
Clin Microbiol Infect. 2019 Jun;25(6):747-752. (PMID: 30321604)
Am J Ophthalmol. 2012 Jun;153(6):1031-7.e2. (PMID: 22381364)
Sci Rep. 2019 Jan 29;9(1):844. (PMID: 30696908)
Ophthalmic Surg Lasers Imaging Retina. 2023 Dec;54(12):720-722. (PMID: 38113358)
Pathology. 2020 Aug;52(5):584-588. (PMID: 32576387)
Diagn Microbiol Infect Dis. 2019 Mar;93(3):232-237. (PMID: 30509499)
J Clin Microbiol. 2013 Mar;51(3):752-8. (PMID: 23254127)
J Clin Microbiol. 2022 May 18;60(5):e0243721. (PMID: 35400176)
Invest Ophthalmol Vis Sci. 2008 May;49(5):1971-8. (PMID: 18436828)
Eur J Clin Microbiol Infect Dis. 2019 Oct;38(10):1873-1881. (PMID: 31313101)
Curr Ophthalmol Rep. 2013 Dec 1;1(4):. (PMID: 24416712)
Arch Ophthalmol. 1997 Sep;115(9):1142-50. (PMID: 9298055)
فهرسة مساهمة: Keywords: Chorioretinitis; Endophthalmitis; Intraocular infection; Next-generation sequencing; Quantitative PCR; pan-PCR
تواريخ الأحداث: Date Created: 20240801 Latest Revision: 20240804
رمز التحديث: 20240804
مُعرف محوري في PubMed: PMC11294505
DOI: 10.1186/s12348-024-00419-9
PMID: 39088113
قاعدة البيانات: MEDLINE
الوصف
تدمد:1869-5760
DOI:10.1186/s12348-024-00419-9