دورية أكاديمية
Fabrication and characterization of calcium peroxide and berberine loaded cryogels for enhanced wound healing.
| العنوان: | Fabrication and characterization of calcium peroxide and berberine loaded cryogels for enhanced wound healing. |
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| المؤلفون: | Dar LA; Division of Animal Biotechnology, Faculty of Veterinary Sciences & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Shuhama Srinagar, Jammu and Kashmir, 190006, India. mudasirbio@gmail.com.; Department of Pharmaceutical Sciences, University of Kashmir, Hazratbal Srinagar, Jammu and Kashmir, 190006, India., Manzoor T; Division of Animal Biotechnology, Faculty of Veterinary Sciences & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Shuhama Srinagar, Jammu and Kashmir, 190006, India. mudasirbio@gmail.com., Shafi S; Department of Pharmaceutical Sciences, University of Kashmir, Hazratbal Srinagar, Jammu and Kashmir, 190006, India., Kumar A; Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India., Ahmad SM; Division of Animal Biotechnology, Faculty of Veterinary Sciences & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Shuhama Srinagar, Jammu and Kashmir, 190006, India. mudasirbio@gmail.com. |
| المصدر: | Journal of materials chemistry. B [J Mater Chem B] 2024 Aug 28; Vol. 12 (34), pp. 8431-8443. Date of Electronic Publication: 2024 Aug 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101598493 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-7518 (Electronic) Linking ISSN: 2050750X NLM ISO Abbreviation: J Mater Chem B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Berberine*/chemistry , Berberine*/pharmacology , Wound Healing*/drug effects , Cryogels*/chemistry , Peroxides*/chemistry , Peroxides*/pharmacology, Animals ; Mice ; Rats ; NIH 3T3 Cells ; Cell Proliferation/drug effects ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Biocompatible Materials/chemical synthesis ; Rats, Sprague-Dawley ; Male ; Porosity |
| مستخلص: | Wound healing represents a complex biological process crucial for tissue repair and regeneration. In recent years, biomaterial-based scaffolds loaded with bioactive compounds have emerged as promising therapeutic strategies to accelerate wound healing. In this study, we investigated the properties and wound healing effects of cryogels loaded with calcium peroxide (CP) and berberine (BB). The cryogels were synthesized through a cryogenic freezing technique and displayed pore diameters of 83 ± 39 μm, with porosity exceeding 90%. Following 20 days of degradation, the percentage of remaining weight for GPC and GPC-CP-BB cryogels was determined to be 12.42 ± 2.45% and 10.78 ± 2.08%, respectively. Moreover, the swelling ratios after 3 minutes for GPC and GPC-CP-BB were found to be 22.10 ± 0.05 and 21.00 ± 0.07, respectively. In vitro investigations demonstrated the cytocompatibility of the cryogels, with sufficient adhesion and proliferation of fibroblast (NIH-3T3) cells observed on the scaffolds, along with their hemocompatibility. Furthermore, the cryogels exhibited sustained release kinetics of both calcium peroxide and berberine, ensuring prolonged therapeutic effects at the wound site. In vivo assessment using a rat model of full-thickness skin wounds demonstrated accelerated wound closure rates in animals treated with the GPC-CP-BB scaffold compared to controls. Histological analysis revealed enhanced granulation tissue formation, re-epithelialization, and collagen deposition in the GPC-CP-BB group. Overall, our findings suggest that the scaffold loaded with CP and BB holds great promise as a therapeutic approach for promoting wound healing. Its multifaceted properties offer a multifunctional platform for localized delivery of therapeutic agents while providing mechanical support and maintaining a favorable microenvironment for tissue regeneration. |
| المشرفين على المادة: | 0I8Y3P32UF (Berberine) 0 (Cryogels) 7FRO2ENO91 (calcium peroxide) 0 (Peroxides) 0 (Biocompatible Materials) |
| تواريخ الأحداث: | Date Created: 20240805 Date Completed: 20240828 Latest Revision: 20240828 |
| رمز التحديث: | 20240830 |
| DOI: | 10.1039/d4tb00989d |
| PMID: | 39101879 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2050-7518 |
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| DOI: | 10.1039/d4tb00989d |