دورية أكاديمية
Proximity labeling expansion microscopy (PL-ExM) evaluates interactome labeling techniques.
| العنوان: | Proximity labeling expansion microscopy (PL-ExM) evaluates interactome labeling techniques. |
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| المؤلفون: | Park S; Center for Complex Biological Systems, University of California, Irvine, Irvine, CA 92697, USA. xiaoyu.shi@uci.edu., Wang X; Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697, USA., Mo Y; Center for Complex Biological Systems, University of California, Irvine, Irvine, CA 92697, USA. xiaoyu.shi@uci.edu., Zhang S; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA., Li X; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94143, USA., Fong KC; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA., Yu C; Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697, USA., Tran AA; Cardiovascular Research Institute, School of Medicine, University of California, San Francisco, San Francisco 94143, USA., Scipioni L; Laboratory for Fluorescence Dynamics, University of California, Irvine, Irvine, CA 92697, USA.; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA., Dai Z; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94143, USA., Huang X; School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USA., Huang L; Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697, USA., Shi X; Center for Complex Biological Systems, University of California, Irvine, Irvine, CA 92697, USA. xiaoyu.shi@uci.edu.; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA.; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.; Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA. |
| المصدر: | Journal of materials chemistry. B [J Mater Chem B] 2024 Aug 06. Date of Electronic Publication: 2024 Aug 06. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101598493 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2050-7518 (Electronic) Linking ISSN: 2050750X NLM ISO Abbreviation: J Mater Chem B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مستخلص: | Understanding protein-protein interactions (PPIs) through proximity labeling has revolutionized our comprehension of cellular mechanisms and pathology. Various proximity labeling techniques, such as HRP, APEX, BioID, TurboID, and μMap, have been widely used to biotinylate PPIs or organelles for proteomic profiling. However, the variability in labeling precision and efficiency of these techniques often results in limited reproducibility in proteomic detection. We address this persistent challenge by introducing proximity labeling expansion microscopy (PL-ExM), a super-resolution imaging technique that combines expansion microscopy with proximity labeling techniques. PL-ExM enabled up to 17 nm resolution with microscopes widely available, providing visual comparison of the labeling precision, efficiency, and false positives of different proximity labeling methods. Our mass spectrometry proteomic results confirmed that PL-ExM imaging is reliable in guiding the selection of proximity labeling techniques and interpreting the proteomic results with new spatial information. |
| تواريخ الأحداث: | Date Created: 20240806 Latest Revision: 20240806 |
| رمز التحديث: | 20240806 |
| DOI: | 10.1039/d4tb00516c |
| PMID: | 39105364 |
| قاعدة البيانات: | MEDLINE |
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