دورية أكاديمية
A multitissue transcriptomic analysis reveals a potential mechanism whereby Brevibacillus laterosporus S62-9 promotes broiler growth.
| العنوان: | A multitissue transcriptomic analysis reveals a potential mechanism whereby Brevibacillus laterosporus S62-9 promotes broiler growth. |
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| المؤلفون: | Zhi T; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China., Ma A; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China., Liu X; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China., Chen Z; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China., Li S; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China., Jia Y; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. Electronic address: jiayingmin@btbu.edu.cn. |
| المصدر: | Poultry science [Poult Sci] 2024 Oct; Vol. 103 (10), pp. 104050. Date of Electronic Publication: 2024 Jun 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Cambridge, UK] : Elsevier Original Publication: Champaign Il : Poultry Science Association |
| مواضيع طبية MeSH: | Chickens*/growth & development , Chickens*/genetics , Animal Feed*/analysis , Brevibacillus*/genetics , Diet*/veterinary , Gene Expression Profiling*/veterinary, Animals ; Probiotics/administration & dosage ; Probiotics/pharmacology ; Transcriptome ; Lipid Metabolism ; Random Allocation ; Dietary Supplements/analysis |
| مستخلص: | Brevibacillus laterosporus S62-9 has been shown to improve broiler growth performance and immunity. In the present study, we aimed to evaluate the effects of B. laterosporus S62-9 on the immunity and lipid metabolism of broilers by means of transcriptomic analysis. A total of 160 1-day-old broilers were randomly allocated to a S62-9 group, the diet of which was supplemented with 10 6 CFU/g B. laterosporus S62-9 daily, and a control group, which was not. After 42 d of feeding, the broilers in the S62-9 group had higher body mass (7.2%) and feed conversion ratio (5.19%) than the control group. Supplementation with B. laterosporus S62-9 resulted in lower serum total cholesterol and low-density lipoprotein-cholesterol concentrations and higher high-density lipoprotein-cholesterol concentrations. An analysis of the fatty acid composition of the broiler's thigh muscles revealed that the proportions of the unsaturated fatty acids myristoleic acid (C14:1) and arachidonic acid (C20:1) were higher for birds in the S62-9 group. Transcriptomic analysis also showed an upregulation of immunity-related genes in the S62-9 group. Gene Ontology functional enrichment analysis showed that the mitogen-activated protein kinase pathway was enriched in the liver, the defense response was enriched in the duodenum, and immunoglobulin-related entries were enriched in the jejunum of the S62-9 group. Furthermore, the expression of key genes involved in unsaturated fatty acid synthesis (SCD, encoding stearoyl-CoA desaturase) and fatty acid metabolism (HACD2, encoding 3-hydroxyacyl-CoA dehydratase 2) was upregulated in the liver, and the expression of genes associated with fat biosynthesis and accumulation, such as PLIN1, encoding perilipin 1, and FABP4, encoding fatty acid binding protein 4, was upregulated in the ileum of the birds in the S62-9 group. In summary, supplementation with B. laterosporus S62-9 could improve immune defense and the fatty acid metabolism of broiler chickens, thereby enhancing their disease resistance and promoting growth and development. Competing Interests: DISCLOSURES The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Brevibacillus laterosporus S62-9; broiler; feed additive; lipid metabolism; transcriptome |
| تواريخ الأحداث: | Date Created: 20240806 Date Completed: 20240921 Latest Revision: 20240921 |
| رمز التحديث: | 20240922 |
| مُعرف محوري في PubMed: | PMC11343061 |
| DOI: | 10.1016/j.psj.2024.104050 |
| PMID: | 39106700 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-3171 |
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| DOI: | 10.1016/j.psj.2024.104050 |