دورية أكاديمية
Meta-genomic next-generation sequencing in the diagnosis of brucellosis: Five cases from a non-endemic area.
| العنوان: | Meta-genomic next-generation sequencing in the diagnosis of brucellosis: Five cases from a non-endemic area. |
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| المؤلفون: | Zhang X; State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Yao X; State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Chen H; State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Han D; Department of Laboratory Medicine, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, Zhejiang, China.; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, Zhejiang, China., Yang M; State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. |
| المصدر: | Future microbiology [Future Microbiol] 2024 Sep; Vol. 19 (13), pp. 1111-1117. Date of Electronic Publication: 2024 Aug 07. |
| نوع المنشور: | Journal Article; Case Reports |
| اللغة: | English |
| بيانات الدورية: | Publisher: Future Medicine Country of Publication: England NLM ID: 101278120 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1746-0921 (Electronic) Linking ISSN: 17460913 NLM ISO Abbreviation: Future Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Future Medicine |
| مواضيع طبية MeSH: | Brucellosis*/diagnosis , Brucellosis*/microbiology , High-Throughput Nucleotide Sequencing* , Brucella melitensis*/genetics , Brucella melitensis*/isolation & purification, Humans ; Male ; Female ; Middle Aged ; Adult ; Metagenomics/methods ; Anti-Bacterial Agents/therapeutic use ; Anti-Bacterial Agents/pharmacology |
| مستخلص: | Metagenomic next-generation sequencing (mNGS) in diagnosis of human brucellosis is comparatively unexplored. This report details five human brucellosis cases diagnosed using mNGS based on Illumina sequencing platform, comprising three females and two males, four with epidemiological exposure. In cases 1 and 2, plasma mNGS results showed one positive and one negative for Brucella melitensis , and subsequent blood cultures were both positive. Cases 3, 4 and 5 involved spinal brucellosis, some with paravertebral abscesses. mNGS from infectious tissue samples successfully detected Brucella , with read counts ranging between 30 and 1314, yet cultures were negative in cases 4 and 5. Following antibiotic and surgical treatments, all patients showed clinical improvement. This report shows mNGS testing enhances the detection sensitivity of brucellosis diagnosis. |
| معلومات مُعتمدة: | 2022YFC2304500, 2022 YFC2304505 National Key R&D Program of China; LY23H200001 Zhejiang Province Natural Science Foundation |
| فهرسة مساهمة: | Keywords: Brucella melitensis; brucellosis; diagnosis; meta-genomic next-generation sequencing (mNGS); spinal brucellosis Local Abstract: [plain-language-summary] What is this summary about? Brucella is a type of bacteria that can infect humans and animals. It causes a disease called brucellosis. Symptoms of brucellosis include fever and fatigue, among others. Meta-genomic next-generation sequencing (mNGS) is a tool for sequencing the DNA of bacteria. In this report, we use mNGS to diagnose human brucellosis in five cases.What were the results? Brucella was found in the blood of two infected people, but mNGS found Brucella in only one. Of three people with Brucella infection of the spine, mNGS found Brucella in the infected tissue but Brucella was only cultured in one case. Following antibiotic and surgical treatments, all five patients showed improvement of their symptoms.What do the results of the study mean? mNGS is a relatively rapid and effective diagnostic method that can improve the detection of Brucella in brucellosis. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20240807 Date Completed: 20240816 Latest Revision: 20240816 |
| رمز التحديث: | 20240816 |
| DOI: | 10.1080/17460913.2024.2363632 |
| PMID: | 39109822 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1746-0921 |
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| DOI: | 10.1080/17460913.2024.2363632 |