دورية أكاديمية
Bioinspired oriented calcium phosphate nanocrystal arrays with bactericidal and osteogenic properties.
| العنوان: | Bioinspired oriented calcium phosphate nanocrystal arrays with bactericidal and osteogenic properties. |
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| المؤلفون: | Degli Esposti L; Institute of Science, Technology and Sustainability for Ceramics (ISSMC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy., Squitieri D; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Fusacchia C; Institute of Science, Technology and Sustainability for Ceramics (ISSMC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy; Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Via delle Scienze 11/A, 43124, Parma (PR), Italy., Bassi G; Institute of Science, Technology and Sustainability for Ceramics (ISSMC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy; Department of Neuroscience, Imaging and Clinical Science. University of G. d'Annunzio, Via dei Vestini 31, 66100, Chieti, Italy., Torelli R; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Altamura D; Istituto di Cristallografia, Consiglio Nazionale delle Ricerche (CNR), Via Amendola 122/O, 70126 Bari, Italy., Manicone E; Istituto di Cristallografia, Consiglio Nazionale delle Ricerche (CNR), Via Amendola 122/O, 70126 Bari, Italy; Dipartimento di Chimica, Università degli studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy., Panseri S; Institute of Science, Technology and Sustainability for Ceramics (ISSMC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy., Adamiano A; Institute of Science, Technology and Sustainability for Ceramics (ISSMC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy., Giannini C; Istituto di Cristallografia, Consiglio Nazionale delle Ricerche (CNR), Via Amendola 122/O, 70126 Bari, Italy., Montesi M; Institute of Science, Technology and Sustainability for Ceramics (ISSMC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy., Bugli F; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. Electronic address: francesca.bugli@unicatt.it., Iafisco M; Institute of Science, Technology and Sustainability for Ceramics (ISSMC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy. Electronic address: michele.iafisco@issmc.cnr.it. |
| المصدر: | Acta biomaterialia [Acta Biomater] 2024 Sep 15; Vol. 186, pp. 470-488. Date of Electronic Publication: 2024 Aug 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101233144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-7568 (Electronic) Linking ISSN: 17427061 NLM ISO Abbreviation: Acta Biomater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Kidlington, Oxford, UK : Elsevier, c2004- |
| مواضيع طبية MeSH: | Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry , Calcium Phosphates*/chemistry , Calcium Phosphates*/pharmacology , Osteogenesis*/drug effects , Nanoparticles*/chemistry , Mesenchymal Stem Cells*/cytology , Mesenchymal Stem Cells*/metabolism , Mesenchymal Stem Cells*/drug effects , Staphylococcus aureus*/drug effects, Humans ; Pseudomonas aeruginosa/drug effects ; Cell Differentiation/drug effects ; Biomimetic Materials/chemistry ; Biomimetic Materials/pharmacology |
| مستخلص: | The global diffusion of antibiotic resistance poses a severe threat to public health. Addressing antibiotic-resistant infections requires innovative approaches, such as antibacterial nanostructured surfaces (ANSs). These surfaces, featuring ordered arrays of nanostructures, exhibit the ability to kill bacteria upon contact. However, most currently developed ANSs utilize bioinert materials, lacking bioactivity crucial for promoting tissue regeneration, particularly in the context of bone infections. This study introduces ANSs composed of bioactive calcium phosphate nanocrystals. Two distinct ANSs were created through a biomineralization-inspired growth of amorphous calcium phosphate (ACP) precursors. The ANSs demonstrated efficient antibacterial properties against both Gram-negative (P. aeruginosa) and Gram-positive (S. aureus) antibiotic resistant bacteria, with up to 75 % mortality in adhered bacteria after only 4 h of contact. Notably, the ANS featuring thinner and less oriented nano-needles exhibited superior efficacy attributed to simultaneous membrane rupturing and oxidative stress induction. Moreover, the ANSs facilitate the proliferation of mammalian cells, enhancing adhesion, spreading, and reducing oxidative stress. The ANSs displayed also significant bioactivity towards human mesenchymal stem cells, promoting colonization and inducing osteogenic differentiation. Specifically, the ANS with thicker and more ordered nano-needles demonstrated heightened effects. In conclusion, ANSs introduced in this work have the potential to serve as foundation for developing bone graft materials capable of eradicate site infections while concurrently stimulating bone regeneration. STATEMENT OF SIGNIFICANCE: Nanostructured surfaces with antibacterial properties through a mechano-bactericidal mechanism have shown significant potential in fighting antibiotic resistance. However, these surfaces have not been fabricated with bioactive materials necessary for developing devices that are both antibacterial and able to stimulate tissue regeneration. This study demonstrates the feasibility of creating nanostructured surfaces of ordered calcium phosphate nano-needles through a biomineralization-inspired growth. These surfaces exhibit dual functionality, serving as effective bactericidal agents against Gram-negative and Gram-positive antibiotic-resistant bacteria while also promoting the proliferation of mammalian cells and inducing osteogenic differentiation of human mesenchymal stem cells. Consequently, this approach holds promise in the context of bone infections, introducing innovative nanostructured surfaces that could be utilized in the development of antimicrobial and osteogenic grafts. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibacterial nanostructured surface; Antibiotic resistance; Bio-inspired material; Calcium phosphate; Osteogenic differentiation |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Calcium Phosphates) 97Z1WI3NDX (calcium phosphate) |
| تواريخ الأحداث: | Date Created: 20240808 Date Completed: 20240911 Latest Revision: 20240911 |
| رمز التحديث: | 20240912 |
| DOI: | 10.1016/j.actbio.2024.08.001 |
| PMID: | 39117114 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-7568 |
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| DOI: | 10.1016/j.actbio.2024.08.001 |