دورية أكاديمية
Grafting of cationic molecules to hyaluronic acid improves adsorption and cartilage lubrication.
| العنوان: | Grafting of cationic molecules to hyaluronic acid improves adsorption and cartilage lubrication. |
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| المؤلفون: | Gonzales G; Department of Biomedical Engineering, Duke University, Durham, NC 27710, USA. shyni.varghese@duke.edu.; Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, NC 27710, USA., Hoque J; Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, NC 27710, USA., Kaeo C; Department of Biomedical Engineering, Duke University, Durham, NC 27710, USA. shyni.varghese@duke.edu., Zauscher S; Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27710, USA., Varghese S; Department of Biomedical Engineering, Duke University, Durham, NC 27710, USA. shyni.varghese@duke.edu.; Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, NC 27710, USA.; Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27710, USA. |
| المصدر: | Biomaterials science [Biomater Sci] 2024 Sep 10; Vol. 12 (18), pp. 4747-4758. Date of Electronic Publication: 2024 Sep 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101593571 Publication Model: Electronic Cited Medium: Internet ISSN: 2047-4849 (Electronic) Linking ISSN: 20474830 NLM ISO Abbreviation: Biomater Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Hyaluronic Acid*/chemistry , Hyaluronic Acid*/pharmacology , Cartilage, Articular*/drug effects , Cartilage, Articular*/metabolism , Cations*/chemistry , Lubrication* , Polylysine*/chemistry , Polylysine*/pharmacology, Adsorption ; Animals ; Cattle ; Lubricants/chemistry ; Lubricants/pharmacology ; Friction/drug effects ; Synovial Fluid/metabolism ; Synovial Fluid/chemistry ; Synovial Fluid/drug effects |
| مستخلص: | Synovial fluid lubricates articular joints by forming a hydrated layer between the cartilage surfaces. In degenerative joint diseases like osteoarthritis (OA), the synovial fluid is compromised, which leads to less effective innate lubrication and exacerbated cartilage degeneration. Studies over the years have led to the development of partially or fully synthetic biolubricants to reduce the coefficient of friction with cartilage in knee joints. Cartilage-adhering, hydrated lubricants are particularly important to provide cartilage lubrication and chondroprotection under high normal load and slow speed. Here, we report the development of a hyaluronic acid (HA)-based lubricant functionalized with cationic branched poly-L-lysine (BPL) molecules that bind to cartilage via electrostatic interactions. We surmised that the electrostatic interactions between the BPL-modified HA molecules (HA-BPL) and the cartilage facilitate localization of the HA molecules to the cartilage surface. The number of BPL molecules on the HA backbone was varied to determine the optimal grafting density for cartilage binding and HA localization. Collectively, our results show that our HA-BPL molecules adhered readily to cartilage and were effective as a lubricant in cartilage-on-cartilage shear measurements where the modified HA molecules significantly reduce the coefficient of friction compared to phosphate-buffered saline or HA alone. This proof-of-concept study shows how the incorporation of cartilage adhering moieties, such as cationic molecules, can be used to enhance cartilage binding and lubrication properties of HA. |
| معلومات مُعتمدة: | R01 AR082809 United States AR NIAMS NIH HHS |
| المشرفين على المادة: | 9004-61-9 (Hyaluronic Acid) 0 (Cations) 25104-18-1 (Polylysine) 0 (Lubricants) |
| تواريخ الأحداث: | Date Created: 20240809 Date Completed: 20240910 Latest Revision: 20240923 |
| رمز التحديث: | 20240923 |
| مُعرف محوري في PubMed: | PMC11310657 |
| DOI: | 10.1039/d4bm00532e |
| PMID: | 39118400 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2047-4849 |
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| DOI: | 10.1039/d4bm00532e |