دورية أكاديمية
أعرض في EDS

Unveiling the Differentiation Potential of Ovarian Theca Interna Cells from Multipotent Stem Cell-like Cells.

التفاصيل البيبلوغرافية
العنوان: Unveiling the Differentiation Potential of Ovarian Theca Interna Cells from Multipotent Stem Cell-like Cells.
المؤلفون: Vlieghe H; Pôle de Recherche en Physiopathologie de la Reproduction, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Hippocrate 54, bte B1.55.03, 1200 Brussels, Belgium., Sousa MJ; Pôle de Recherche en Physiopathologie de la Reproduction, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Hippocrate 54, bte B1.55.03, 1200 Brussels, Belgium., Charif D; Pôle de Recherche en Physiopathologie de la Reproduction, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Hippocrate 54, bte B1.55.03, 1200 Brussels, Belgium., Amorim CA; Pôle de Recherche en Physiopathologie de la Reproduction, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Hippocrate 54, bte B1.55.03, 1200 Brussels, Belgium.
المصدر: Cells [Cells] 2024 Jul 25; Vol. 13 (15). Date of Electronic Publication: 2024 Jul 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Cell Differentiation* , Theca Cells*/metabolism , Theca Cells*/cytology, Female ; Humans ; Mesenchymal Stem Cells/metabolism ; Mesenchymal Stem Cells/cytology ; Multipotent Stem Cells/cytology ; Multipotent Stem Cells/metabolism ; Cells, Cultured ; Biomarkers/metabolism ; Receptors, Nerve Growth Factor/metabolism ; Receptors, Nerve Growth Factor/genetics ; Receptor, Platelet-Derived Growth Factor alpha/metabolism ; Receptor, Platelet-Derived Growth Factor alpha/genetics
مستخلص: Research Question: Theca interna cells (TICs) are an indispensable cell source for ovarian follicle development and steroidogenesis. Recent studies have identified theca stem cells (TSCs) in both humans and animals. Interestingly, TSCs express mesenchymal stem cell (MSC)-related markers and can differentiate into mesenchymal lineages. MSCs are promising for tissue engineering and regenerative medicine due to their self-renewal and differentiation abilities. Therefore, this study investigated the potential origin of TICs from MSCs.
Design: Whole ovaries from postmenopausal organ donors were obtained, and their cortex was cryopreserved prior to the isolation of stromal cells. These isolated cells were differentiated in vitro to TICs using cell media enriched with various growth factors and hormones. Immunocytochemistry, an enzyme-linked immunosorbent assay, flow cytometry, and reverse transcription-quantitative polymerase chain were employed at different timepoints. Data were analyzed using one-way ANOVA.
Results: Immunocytochemistry showed an increase in TIC markers from day 0 to day 8 and a significant rise in MSC-like markers on day 2. This corresponds with rising androstenedione levels from day 2 to day 13. Flow cytometry identified a decreasing MSC-like cell population from day 2 onwards. The CD13+ cell population and its gene expression increased significantly over time. NGFR and PDGFRA expression was induced on days 0 and 2, respectively, compared to day 13.
Conclusions: This study offers insights into MSC-like cells as the potential origin of TICs. Differentiating TICs from these widely accessible MSCs holds potential significance for toxicity studies and investigating TIC-related disorders like polycystic ovary syndrome (PCOS).
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معلومات مُعتمدة: T.0004.20 FNRS; IREC FSR22 UCLouvain's Fonds spéciaux de recherche
فهرسة مساهمة: Keywords: differentiation; multipotent mesenchymal stem cells; ovarian follicle development; steroidogenesis; theca interna cells
المشرفين على المادة: 0 (Biomarkers)
0 (Receptors, Nerve Growth Factor)
EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
تواريخ الأحداث: Date Created: 20240809 Date Completed: 20240809 Latest Revision: 20240811
رمز التحديث: 20240812
مُعرف محوري في PubMed: PMC11311681
DOI: 10.3390/cells13151248
PMID: 39120279
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4409
DOI:10.3390/cells13151248