دورية أكاديمية
A Low-Cost Handheld Centrifugal Microfluidic System for Multiplexed Visual Detection Based on Isothermal Amplification.
| العنوان: | A Low-Cost Handheld Centrifugal Microfluidic System for Multiplexed Visual Detection Based on Isothermal Amplification. |
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| المؤلفون: | Wang N; Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China., Dong X; Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China., Zhou Y; Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China., Zhu R; Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China., Liu L; Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China., Zhang L; Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China., Qiu X; Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. |
| المصدر: | Sensors (Basel, Switzerland) [Sensors (Basel)] 2024 Aug 03; Vol. 24 (15). Date of Electronic Publication: 2024 Aug 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101204366 Publication Model: Electronic Cited Medium: Internet ISSN: 1424-8220 (Electronic) Linking ISSN: 14248220 NLM ISO Abbreviation: Sensors (Basel) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c2000- |
| مواضيع طبية MeSH: | Nucleic Acid Amplification Techniques*/instrumentation , Nucleic Acid Amplification Techniques*/methods, African Swine Fever Virus/isolation & purification ; African Swine Fever Virus/genetics ; Lab-On-A-Chip Devices ; Limit of Detection ; Centrifugation/instrumentation ; Animals ; Smartphone ; Biosensing Techniques/instrumentation ; Biosensing Techniques/methods ; Microfluidic Analytical Techniques/instrumentation ; Microfluidic Analytical Techniques/methods ; Microfluidic Analytical Techniques/economics |
| مستخلص: | A low-cost, handheld centrifugal microfluidic system for multiplexed visual detection based on recombinase polymerase amplification (RPA) was developed. A concise centrifugal microfluidic chip featuring four reaction units was developed to run multiplexed RPA amplification in parallel. Additionally, a significantly shrunk-size and cost-effective handheld companion device was developed, incorporating heating, optical, rotation, and sensing modules, to perform multiplexed amplification and visual detection. After one-time sample loading, the metered sample was equally distributed into four separate reactors with high-speed centrifugation. Non-contact heating was adopted for isothermal amplification. A tiny DC motor on top of the chip was used to drive steel beads inside reactors for active mixing. Another small DC motor, which was controlled by an elaborate locking strategy based on magnetic sensing, was adopted for centrifugation and positioning. Visual fluorescence detection was optimized from different sides, including material, surface properties, excitation light, and optical filters. With fluorescence intensity-based visual detection, the detection results could be directly observed through the eyes or with a smartphone. As a proof of concept, the handheld device could detect multiple targets, e.g., different genes of African swine fever virus (ASFV) with the comparable LOD (limit of detection) of 75 copies/test compared to the tube-based RPA. |
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| معلومات مُعتمدة: | NO. 81871505 National Natural Science Foundation of China; NO. 61971026 National Natural Science Foundation of China; No. buctylkjcx06 Research fund to the top scientific and technological innovation team from Beijing University of Chemical Technology, China |
| فهرسة مساهمة: | Keywords: RPA; centrifugal microfluidic; handheld; multiplexed; visual detection |
| تواريخ الأحداث: | Date Created: 20240810 Date Completed: 20240810 Latest Revision: 20240812 |
| رمز التحديث: | 20240813 |
| مُعرف محوري في PubMed: | PMC11314988 |
| DOI: | 10.3390/s24155028 |
| PMID: | 39124075 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1424-8220 |
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| DOI: | 10.3390/s24155028 |