دورية أكاديمية
أعرض في EDS

Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy.

التفاصيل البيبلوغرافية
العنوان: Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy.
المؤلفون: Fang Y; Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 9 Jinsui Road, Tianhe District, Guangzhou, Guangdong Province, 510623, China., Kang Z; Department of Pediatric Orthopedics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, 510623, China., Zhang W; Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 9 Jinsui Road, Tianhe District, Guangzhou, Guangdong Province, 510623, China., Xiang Y; Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 9 Jinsui Road, Tianhe District, Guangzhou, Guangdong Province, 510623, China., Cheng X; Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 9 Jinsui Road, Tianhe District, Guangzhou, Guangdong Province, 510623, China., Gui M; Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 9 Jinsui Road, Tianhe District, Guangzhou, Guangdong Province, 510623, China., Fang D; Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 9 Jinsui Road, Tianhe District, Guangzhou, Guangdong Province, 510623, China. fangdajun017@163.com.
المصدر: BMC pregnancy and childbirth [BMC Pregnancy Childbirth] 2024 Aug 10; Vol. 24 (1), pp. 525. Date of Electronic Publication: 2024 Aug 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967799 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2393 (Electronic) Linking ISSN: 14712393 NLM ISO Abbreviation: BMC Pregnancy Childbirth Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Cholestasis, Intrahepatic*/metabolism , Cholestasis, Intrahepatic*/diagnosis , Pregnancy Complications*/metabolism , Pregnancy Complications*/diagnosis , Biomarkers*/metabolism , Biomarkers*/analysis , Proteomics*/methods , Bile Acids and Salts*/metabolism , Placenta*/metabolism , Metabolomics*/methods, Female ; Humans ; Pregnancy ; Rats ; Animals ; Adult ; Disease Models, Animal ; Tandem Mass Spectrometry
مستخلص: Background: The pregnant women with intrahepatic cholestasis were at high risk of fetal distress, preterm birth and unexpected stillbirth. Intrahepatic cholestasis of pregnancy (ICP) was mainly caused by disorder of bile acid metabolism, whereas the specific mechanism was obscure.
Methods: We performed proteomics analysis of 10 ICP specimens and 10 placenta specimens from patients without ICP through data-independent acquisition (DIA) technique to disclose differentially expressed proteins. We executed metabolomic analysis of 30 ICP specimens and 30 placenta specimens from patients without ICP through UPLC-MS/MS to identify differentially expressed metabolites. Enrichment and correlation analysis was used to obtain the direct molecular insights of ICP development. The ICP rat models were constructed to validate pathological features.
Results: The heatmap of proteomics analysis showed the top 30 up-regulated and 30 down-regulated proteins. The metabolomic analysis revealed 20 richer and 4 less abundant metabolites in ICP samples compared with placenta specimens from patients without ICP, and enrichment pathways by these metabolites included primary bile acid biosynthesis, cholesterol metabolism, bile secretion, nicotinate and nicotinamide metabolism, purine metabolism and metabolic pathways. Combined analysis of multiple omics results demonstrated that bile acids such as Glycohyocholic acid, Glycine deoxycholic acid, beta-Muricholic acid, Noncholic acid, cholic acid, Gamma-Mercholic Acid, alpha-Muricholic acid and Glycochenodeoxycholic Aicd were significantly associated with the expression of GLRX3, MYL1, MYH7, PGGT1B, ACTG1, SP3, LACTB2, C2CD5, APBB2, IPO9, MYH2, PPP3CC, PIN1, BLOC1S1, DNAJC7, RASAL2 and ATCN3 etc. The core protein ACAT2 was involved in lipid metabolic process and animal model showed that ACAT2 was up-regulated in placenta and liver of pregnant rats and fetal rats. The neonates had low birth weight and Safranin O-Fast green FCF staining of animal models showed that poor osteogenic and chondrogenic differentiation of fetal rats.
Conclusion: Multiple metabolites-alpha-Muricholic acid, beta-Muricholic acid, Glycine deoxycholic acid and Glycochenodeoxycholic Acid etc. were perfect biomarkers to predict occurrence of ICP. Bile acids were significantly associated with varieties of protein expression and these proteins were differentially expressed in ICP samples. Our study provided several biomarkers for ICP detection and potential therapeutic targets for ICP development.
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Biomarkers; Intrahepatic cholestasis of pregnancy; Metabolomic analysis; Quantitative proteomics; Target therapy
المشرفين على المادة: 0 (Biomarkers)
0 (Bile Acids and Salts)
SCR Disease Name: Intrahepatic Cholestasis of Pregnancy
تواريخ الأحداث: Date Created: 20240810 Date Completed: 20240810 Latest Revision: 20240813
رمز التحديث: 20240813
مُعرف محوري في PubMed: PMC11317000
DOI: 10.1186/s12884-024-06730-6
PMID: 39127651
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2393
DOI:10.1186/s12884-024-06730-6