دورية أكاديمية
Ase1 selectively increases the lifetime of antiparallel microtubule overlaps.
| العنوان: | Ase1 selectively increases the lifetime of antiparallel microtubule overlaps. |
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| المؤلفون: | Krattenmacher J; Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Czechia; B CUBE - Center of Molecular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany; Faculty of Science, Charles University in Prague, 12800 Prague, Czech Republic., Lera-Ramirez M; Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR144, Paris, France., Beber A; Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Czechia., Herynek S; Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Czechia., Grycova L; Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Czechia., Liu X; School of Mechanical Engineering, Department of Microsystem Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, P.R. China., Neuzil P; School of Mechanical Engineering, Department of Microsystem Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, P.R. China., Nedelec F; Sainsbury Laboratory Cambridge, University of Cambridge, CB2 1LR Cambridge, UK. Electronic address: fjn28@cam.ac.uk., Diez S; B CUBE - Center of Molecular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; Cluster of Excellence Physics of Life, Technische Universität Dresden, 01062 Dresden, Germany. Electronic address: stefan.diez@tu-dresden.de., Braun M; Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Czechia. Electronic address: marcus.braun@ibt.cas.cz., Lansky Z; Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Czechia. Electronic address: zdenek.lansky@ibt.cas.cz. |
| المصدر: | Current biology : CB [Curr Biol] 2024 Sep 09; Vol. 34 (17), pp. 4071-4080.e6. Date of Electronic Publication: 2024 Aug 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: England NLM ID: 9107782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0445 (Electronic) Linking ISSN: 09609822 NLM ISO Abbreviation: Curr Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: London, UK : Current Biology Ltd., c1991- |
| مواضيع طبية MeSH: | Microtubules*/metabolism , Microtubule-Associated Proteins*/metabolism , Microtubule-Associated Proteins*/genetics, Spindle Apparatus/metabolism ; Animals ; Saccharomyces cerevisiae Proteins/metabolism ; Saccharomyces cerevisiae Proteins/genetics |
| مستخلص: | Microtubules (MTs) are dynamically unstable polar biopolymers switching between periods of polymerization and depolymerization, with the switch from the polymerization to the depolymerization phase termed catastrophe and the reverse transition termed rescue. 1 In presence of MT-crosslinking proteins, MTs form parallel or anti-parallel overlaps and self-assemble reversibly into complex networks, such as the mitotic spindle. Differential regulation of MT dynamics in parallel and anti-parallel overlaps is critical for the self-assembly of these networks. 2 , 3 Diffusible MT crosslinkers of the Ase1/MAP65/PRC1 family associate with different affinities to parallel and antiparallel MT overlaps, providing a basis for this differential regulation. 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 Ase1/MAP65/PRC1 family proteins directly affect MT dynamics 12 and recruit other proteins that locally alter MT dynamics, such as CLASP or kinesin-4. 7 , 13 , 14 , 15 , 16 However, how Ase1 differentially regulates MT stability in parallel and antiparallel bundles is unknown. Here, we show that Ase1 selectively promotes antiparallel MT overlap longevity by slowing down the depolymerization velocity and by increasing the rescue frequency, specifically in antiparallelly crosslinked MTs. At the retracting ends of depolymerizing MTs, concomitant with slower depolymerization, we observe retention and accumulation of Ase1 between crosslinked MTs and on isolated MTs. We hypothesize that the ability of Ase1 to reduce the dissociation of tubulin subunits is sufficient to promote its enrichment at MT ends. A mathematical model built on this idea shows good agreement with the experiments. We propose that differential regulation of MT dynamics by Ase1 contributes to mitotic spindle assembly by specifically stabilizing antiparallel overlaps, compared to parallel overlaps or isolated MTs. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Ase1/PRC1/MAP65 crosslinkers; diffusible microtubule crosslinkers; microtubule arrays; microtubule dynamics; microtubule overlap stability; microtubules |
| المشرفين على المادة: | 0 (Microtubule-Associated Proteins) 0 (Saccharomyces cerevisiae Proteins) 0 (Ase1 protein, S cerevisiae) |
| تواريخ الأحداث: | Date Created: 20240813 Date Completed: 20240910 Latest Revision: 20240911 |
| رمز التحديث: | 20240912 |
| DOI: | 10.1016/j.cub.2024.07.055 |
| PMID: | 39137787 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1879-0445 |
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| DOI: | 10.1016/j.cub.2024.07.055 |