دورية أكاديمية
أعرض في EDS

Comparative transcriptomic and metabolomic analyses provide insights into the responses to high temperature stress in Alfalfa (Medicago sativa L.).

التفاصيل البيبلوغرافية
العنوان: Comparative transcriptomic and metabolomic analyses provide insights into the responses to high temperature stress in Alfalfa (Medicago sativa L.).
المؤلفون: Zhou J; College of Forestry and Prataculture, Ningxia University, Yinchuan, 750021, China., Tang X; College of Enology and Horticulture, Ningxia University, Yinchuan, Ningxia, 750021, China., Li J; College of Enology and Horticulture, Ningxia University, Yinchuan, Ningxia, 750021, China., Dang S; College of Enology and Horticulture, Ningxia University, Yinchuan, Ningxia, 750021, China., Ma H; College of Enology and Horticulture, Ningxia University, Yinchuan, Ningxia, 750021, China., Zhang Y; College of Enology and Horticulture, Ningxia University, Yinchuan, Ningxia, 750021, China. zhyhcau@sina.com.
المصدر: BMC plant biology [BMC Plant Biol] 2024 Aug 15; Vol. 24 (1), pp. 776. Date of Electronic Publication: 2024 Aug 15.
نوع المنشور: Journal Article; Comparative Study
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Medicago sativa*/genetics , Medicago sativa*/metabolism , Medicago sativa*/physiology , Transcriptome* , Metabolomics*, Gene Expression Profiling ; Metabolome ; Gene Expression Regulation, Plant ; Hot Temperature ; Stress, Physiological/genetics ; Seedlings/genetics ; Seedlings/metabolism ; Seedlings/physiology ; Seedlings/growth & development ; Heat-Shock Response/genetics
مستخلص: High temperature stress is one of the most severe forms of abiotic stress in alfalfa. With the intensification of climate change, the frequency of high temperature stress will further increase in the future, which will bring challenges to the growth and development of alfalfa. Therefore, untargeted metabolomic and RNA-Seq profiling were implemented to unravel the possible alteration in alfalfa seedlings subjected to different temperature stress (25 ℃, 30 ℃, 35 ℃, 40 ℃) in this study. Results revealed that High temperature stress significantly altered some pivotal transcripts and metabolites. The number of differentially expressed genes (DEGs) markedly up and down-regulated was 1876 and 1524 in T30_vs_CK, 2, 815 and 2667 in T35_vs_CK, and 2115 and 2, 226 in T40_vs_CK, respectively. The number for significantly up-regulated and down-regulated differential metabolites was 173 and 73 in T30_vs_CK, 188 and 57 in T35_vs_CK, and 220 and 66 in T40_vs_CK, respectively. It is worth noting that metabolomics and transcriptomics co-analysis characterized enriched in plant hormone signal transduction (ko04705), glyoxylate and dicarboxylate metabolism (ko00630), from which some differentially expressed genes and differential metabolites participated. In particular, the content of hormone changed significantly under T40 stress, suggesting that maintaining normal hormone synthesis and metabolism may be an important way to improve the HTS tolerance of alfalfa. The qRT-PCR further showed that the expression pattern was similar to the expression abundance in the transcriptome. This study provides a practical and in-depth perspective from transcriptomics and metabolomics in investigating the effects conferred by temperature on plant growth and development, which provided the theoretical basis for breeding heat-resistant alfalfa.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 2021BEF02016 Science and Technology Department of Ningxia Province; 2021BEF02016 Science and Technology Department of Ningxia Province; 2021BEF02016 Science and Technology Department of Ningxia Province; 2021BEF02016 Science and Technology Department of Ningxia Province; 2023BCF1054 Region key research and development; 2023BCF1054 Region key research and development; 2023BCF1054 Region key research and development; 2023BCF1054 Region key research and development
فهرسة مساهمة: Keywords: Alfalfa; High temperature stress; Metabolomic; Transcriptomic
تواريخ الأحداث: Date Created: 20240814 Date Completed: 20240814 Latest Revision: 20240817
رمز التحديث: 20240817
مُعرف محوري في PubMed: PMC11325607
DOI: 10.1186/s12870-024-05494-7
PMID: 39143536
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2229
DOI:10.1186/s12870-024-05494-7