دورية أكاديمية
أعرض في EDS

Comparative molecular and conventional cytogenetic analyses of three species of Rhinella (Anura; Bufonidae).

التفاصيل البيبلوغرافية
العنوان: Comparative molecular and conventional cytogenetic analyses of three species of Rhinella (Anura; Bufonidae).
المؤلفون: Silva DSD; Programa de Pós-Graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., de Sousa RPC; Laboratório de Evolução, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil., Vallinoto M; Laboratório de Evolução, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil., Costa Lima MRD; Laboratório de Biologia Molecular, Evolução e Microbiologia, Instituto Federal do Pará, Abaetetuba, Pará, Brazil., Costa RAD; Laboratório de Biologia Molecular, Evolução e Microbiologia, Instituto Federal do Pará, Abaetetuba, Pará, Brazil., Furo IO; Laboratório de Reprodução Animal, Universidade Federal Rural da Amazônia, Parauapebas, Pará, Brazil., Gomes AJB; Laboratório de Biologia Molecular, Evolução e Microbiologia, Instituto Federal do Pará, Abaetetuba, Pará, Brazil., Oliveira EHC; Faculdade de Ciências Naturais, Instituto de Ciências Exatas Naturais e Exatas, Universidade Federal do Pará, Belém, Pará, Brazil.; Laboratório de Citogenômica e Mutagênese Ambiental, Seção de Meio Ambiente, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
المصدر: PloS one [PLoS One] 2024 Aug 15; Vol. 19 (8), pp. e0308785. Date of Electronic Publication: 2024 Aug 15 (Print Publication: 2024).
نوع المنشور: Journal Article; Comparative Study
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: In Situ Hybridization, Fluorescence* , Karyotype* , Cytogenetic Analysis* , Microsatellite Repeats*/genetics, Animals ; Bufonidae/genetics ; Bufonidae/classification ; Female ; RNA, Ribosomal, 18S/genetics ; Telomere/genetics ; Species Specificity ; Chromosome Banding ; Karyotyping ; Male ; DNA, Ribosomal/genetics
مستخلص: The genus Rhinella corresponds to a group of anurans characterized by numerous taxonomic and systemic challenges, leading to their organization into species complexes. Cytogenetic data for this genus thus far are limited to the diploid number and chromosome morphology, which remain highly conserved among the species. In this study, we analyse the karyotypes of three species of the genus Rhinella (Rhinella granulosa, Rhinella margaritifera, and Rhinella marina) using both classical (conventional staining and C-banding) and molecular (FISH-fluorescence in situ hybridization with 18S rDNA, telomeric sequences, and microsatellite probes) cytogenetic approaches. The aim of this study is to provide data that can reveal variations in the distribution of repetitive sequences that can contribute to understanding karyotypic diversification in these species. The results revealed a conserved karyotype across the species, with 2n = 22 and FN = 44, with metacentric and submetacentric chromosomes. C-banding revealed heterochromatic blocks in the pericentromeric region for all species, with a proximal block on the long arms of pairs 3 and 6 in R. marina and on the short arms of pairs 4 and 6 in R. margaritifera. Additionally, 18S rDNA probes hybridized to pair 5 in R. granulosa, to pair 7 in R. marina, and to pair 10 in R. margaritifera. Telomeric sequence probes displayed signals exclusively in the distal region of the chromosomes, while microsatellite DNA probes showed species-specific patterns. These findings indicate that despite a conserved karyotypical macrostructure, chromosomal differences exist among the species due to the accumulation of repetitive sequences. This variation may be attributed to chromosome rearrangements or differential accumulation of these sequences, highlighting the dynamic role of repetitive sequences in the chromosomal evolution of Rhinella species. Ultimately, this study emphasizes the importance of the role of repetitive DNAs in chromosomal rearrangements to elucidate the evolutionary mechanisms leading to independent diversification in the distinct phylogenetic groups of Rhinella.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (RNA, Ribosomal, 18S)
0 (DNA, Ribosomal)
تواريخ الأحداث: Date Created: 20240815 Date Completed: 20240815 Latest Revision: 20240817
رمز التحديث: 20240817
مُعرف محوري في PubMed: PMC11326569
DOI: 10.1371/journal.pone.0308785
PMID: 39146271
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0308785