دورية أكاديمية
Automated immunohistochemistry of intra-epidermal nerve fibres in skin biopsies: A proof-of-concept study.
| العنوان: | Automated immunohistochemistry of intra-epidermal nerve fibres in skin biopsies: A proof-of-concept study. |
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| المؤلفون: | Burgess J; Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK.; Liverpool University Hospitals NHS Foundation Trust, Liverpool, UK., Marshall A; Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK.; Liverpool University Hospitals NHS Foundation Trust, Liverpool, UK., Rapteas L; Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK., Hamill KJ; Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK., Marshall A; Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK.; The Walton Centre NHS Foundation Trust, Liverpool, UK., Malik RA; Weill Cornell Medicine-Qatar, Doha, Qatar., Frank B; The Walton Centre NHS Foundation Trust, Liverpool, UK., Alam U; Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK.; Department of Cardiovascular and Metabolic Medicine, University of Liverpool, Liverpool, UK.; Centre for Biomechanics and Rehabilitation Technologies, Staffordshire University, Stoke-on-Trent, UK. |
| المصدر: | Journal of the peripheral nervous system : JPNS [J Peripher Nerv Syst] 2024 Sep; Vol. 29 (3), pp. 329-338. Date of Electronic Publication: 2024 Aug 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: United States NLM ID: 9704532 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1529-8027 (Electronic) Linking ISSN: 10859489 NLM ISO Abbreviation: J Peripher Nerv Syst Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2010->: Hoboken, NJ Wiley Original Publication: New York, NY : Woodland Publications, c1996- |
| مواضيع طبية MeSH: | Nerve Fibers*/pathology , Immunohistochemistry* , Proof of Concept Study* , Skin*/innervation , Skin*/pathology , Small Fiber Neuropathy*/diagnosis , Small Fiber Neuropathy*/pathology, Humans ; Female ; Male ; Adult ; Middle Aged ; Biopsy ; Epidermis/innervation ; Epidermis/pathology ; Aged ; Ubiquitin Thiolesterase/metabolism ; Ubiquitin Thiolesterase/analysis ; Reproducibility of Results |
| مستخلص: | Aims: To develop a standardised, automated protocol for detecting protein gene product 9.5 (PGP9.5) positive intra-epidermal nerve fibres (IENFs) in skin biopsies, transitioning from the established manual technique to an automated platform. This automated method, although currently intended for research applications, may improve the accessibility of this diagnostic test for small fibre neuropathy in clinical settings. Methods: Skin biopsies (n = 274) from 100 participants (fibromyalgia syndrome n = 62; idiopathic small fibre neuropathy: n = 16; healthy volunteers: n = 22) were processed using an automated immunohistochemistry platform. IENF quantification was performed by blinded examiners, with reliability assessed via a two-way mixed-effects model to evaluate inter- and intra-observer variability. Results: The automated staining system reproduced intra-epidermal nerve fibre density (IENFD) counts consistent with free-floating sections (mean ± standard deviation: free-floating: 5.6 ± 3.4 fibres/mm; automated: 5.9 ± 3.2 fibres/mm). A median difference of 0.3 with a lower bound 95% Confidence Interval (CI) at -0.00005 established non-inferiority against a margin of -0.4 (p = .08). Specifically, the inter-class correlation coefficient (class denotes consistency in measured observations) was 99% (95% CI: 0.9-1), indicating excellent agreement between free-floating and automated methods. The inter- and intra-class coefficient between examiners were both 99% (95% CI: 0.9-0.1) for IENFD, demonstrating high reliability using sections stained using the automated method. Interpretation: Automated immunohistochemistry provides high-throughput reliable and reproducible intra-epidermal nerve fibre quantification. This method, although currently proof-of-concept, for research use only, may be more widely deployed in histopathology laboratories to increase the adoption of IENFD assessment for the diagnosis of peripheral neuropathies. (© 2024 The Author(s). Journal of the Peripheral Nervous System published by Wiley Periodicals LLC on behalf of Peripheral Nerve Society.) |
| التعليقات: | Erratum in: J Peripher Nerv Syst. 2024 Sep 20. doi: 10.1111/jns.12658. (PMID: 39301914) |
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| معلومات مُعتمدة: | Pain Relief Foundation; United Kingdom VAC_ Versus Arthritis |
| فهرسة مساهمة: | Keywords: automated; density; digital pathology; fibromyalgia syndrome; immunohistochemistry; intra‐epidermal nerve fibres; pathology; quantification; small fibre neuropathy |
| المشرفين على المادة: | 0 (UCHL1 protein, human) EC 3.4.19.12 (Ubiquitin Thiolesterase) |
| تواريخ الأحداث: | Date Created: 20240820 Date Completed: 20240903 Latest Revision: 20240920 |
| رمز التحديث: | 20240920 |
| DOI: | 10.1111/jns.12650 |
| PMID: | 39164223 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1529-8027 |
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| DOI: | 10.1111/jns.12650 |