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First Molecular Evidence of Leishmania Infantum in Domestic Cats and Associated Risk Factors from the Black Sea Region of Türkiye.

التفاصيل البيبلوغرافية
العنوان: First Molecular Evidence of Leishmania Infantum in Domestic Cats and Associated Risk Factors from the Black Sea Region of Türkiye.
المؤلفون: Pekmezci D; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey. dkazanci@omu.edu.tr., Yildirim A; Department of Parasitology, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey., Kot ZN; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey., Konacoglu GN; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey., Duzlu O; Department of Parasitology, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey., Yetismis G; Department of Parasitology, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey., Uslu S; Department of Parasitology, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey., Toz S; Department of Parasitology, Faculty of Medicine, University of Ege, Izmir, Turkey., Ozbel Y; Department of Parasitology, Faculty of Medicine, University of Ege, Izmir, Turkey., Inci A; Department of Parasitology, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey., Pekmezci GZ; Preclinical Sciences, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey.
المصدر: Acta parasitologica [Acta Parasitol] 2024 Sep; Vol. 69 (3), pp. 1547-1554. Date of Electronic Publication: 2024 Aug 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2019- : Cham : Springer International Publishing
Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology
مواضيع طبية MeSH: Leishmania infantum*/genetics , Leishmania infantum*/isolation & purification , Cat Diseases*/parasitology , Cat Diseases*/epidemiology, Animals ; Cats ; Risk Factors ; Black Sea ; Female ; Male ; Leishmaniasis, Visceral/veterinary ; Leishmaniasis, Visceral/parasitology ; Leishmaniasis, Visceral/epidemiology ; Real-Time Polymerase Chain Reaction/veterinary ; Prevalence ; DNA, Kinetoplast/genetics ; DNA, Protozoan/genetics
مستخلص: Purpose: The objectives of the present study are to determine the molecular prevalence of Leishmania spp. in the owned domestic cats in the Black Sea Region of Türkiye and analyze the associated risk factors in FeL.
Methods: Conjunctival swabs (CS), blood, demographic, and clinical data were collected from 150 owned cats brought to the Veterinary Teaching Hospital during 2020-2022. Leishmania kinetoplast DNA (kDNA) from CS was screened by TaqMan Real-Time PCR (qPCR) with the genus-specific primers and a probe.
Results: All qPCR positive products were also amplified and sequenced to identify Leishmania species by ITS1 primers. Molecular prevalence of L. infantum found as 12.6% (19/150) in the observed cats in the Black Sea Region of Türkiye. There was a significant difference (p < 0.05) between neutered and intact cats with regarding to L. infantum positivity. Intact cats found to be 0.368 times more prone to be L. infantum-positive (L+). Dermatological lesions were found the most common (26.3%) problems in the L + cats. The median leucocyte count was the only parameter that was found statistically (p < 0.05) lower in the L + group (6.60) than the negative group (L-) (8.96), when comparing the WBC, NEU/LYM, MONO/LYM, EOS/LYM and PLT/LYM values.
Conclusion: This study presented the molecular occurrence of FeL in the Black Sea Region of Türkiye for the first time indicating that the carrier status of the cats makes them alternative reservoirs for possible zoonotic transmission of L. infantum in this zone.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
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فهرسة مساهمة: Keywords: Leishmania Infantum; Conjunctival swap; Feline Leishmaniosis; Risk Factors; Türkiye; qPCR
المشرفين على المادة: 0 (DNA, Kinetoplast)
0 (DNA, Protozoan)
تواريخ الأحداث: Date Created: 20240820 Date Completed: 20240906 Latest Revision: 20240906
رمز التحديث: 20240906
DOI: 10.1007/s11686-024-00885-0
PMID: 39164546
قاعدة البيانات: MEDLINE
الوصف
تدمد:1896-1851
DOI:10.1007/s11686-024-00885-0