دورية أكاديمية
Affinity-independent memory B cell origin of the early antibody-secreting cell response in naive individuals upon SARS-CoV-2 vaccination.
| العنوان: | Affinity-independent memory B cell origin of the early antibody-secreting cell response in naive individuals upon SARS-CoV-2 vaccination. |
|---|---|
| المؤلفون: | Li Z; B Cell Immunology, German Cancer Research Center, Heidelberg 69120, Germany., Obraztsova A; B Cell Immunology, German Cancer Research Center, Heidelberg 69120, Germany; Faculty of Biosciences, University of Heidelberg, Heidelberg 69120, Germany. Electronic address: a.obraztsova@dkfz.de., Shang F; Cellular Immunology, German Cancer Research Center, Heidelberg 69120, Germany; Faculty of Medicine, University of Heidelberg, Heidelberg 69120, Germany., Oludada OE; B Cell Immunology, German Cancer Research Center, Heidelberg 69120, Germany; Faculty of Biosciences, University of Heidelberg, Heidelberg 69120, Germany., Malapit J; B Cell Immunology, German Cancer Research Center, Heidelberg 69120, Germany; Faculty of Biosciences, University of Heidelberg, Heidelberg 69120, Germany., Busch K; Cellular Immunology, German Cancer Research Center, Heidelberg 69120, Germany., van Straaten M; Structural Biology of Infection and Immunity, German Cancer Research Center, Heidelberg 69120, Germany., Stebbins E; Structural Biology of Infection and Immunity, German Cancer Research Center, Heidelberg 69120, Germany., Murugan R; B Cell Immunology, German Cancer Research Center, Heidelberg 69120, Germany., Wardemann H; B Cell Immunology, German Cancer Research Center, Heidelberg 69120, Germany. Electronic address: h.wardemann@dkfz.de. |
| المصدر: | Immunity [Immunity] 2024 Sep 10; Vol. 57 (9), pp. 2191-2201.e5. Date of Electronic Publication: 2024 Aug 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9432918 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4180 (Electronic) Linking ISSN: 10747613 NLM ISO Abbreviation: Immunity Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1994- |
| مواضيع طبية MeSH: | SARS-CoV-2*/immunology , Memory B Cells*/immunology , COVID-19*/immunology , COVID-19*/prevention & control , Antibodies, Viral*/immunology , Spike Glycoprotein, Coronavirus*/immunology , Antibody-Producing Cells*/immunology , COVID-19 Vaccines*/immunology, Humans ; Vaccination ; Antibody Affinity/immunology ; Cell Differentiation/immunology ; Germinal Center/immunology ; Immunologic Memory/immunology ; Antibodies, Monoclonal/immunology ; Adult ; Female |
| مستخلص: | Memory B cells (MBCs) formed over the individual's lifetime constitute nearly half of the circulating B cell repertoire in humans. These pre-existing MBCs dominate recall responses to their cognate antigens, but how they respond to recognition of novel antigens is not well understood. Here, we tracked the origin and followed the differentiation paths of MBCs in the early anti-spike (S) response to mRNA vaccination in SARS-CoV-2-naive individuals on single-cell and monoclonal antibody levels. Pre-existing, highly mutated MBCs showed no signs of germinal center re-entry and rapidly developed into mature antibody-secreting cells (ASCs). By contrast, and despite similar levels of S reactivity, naive B cells showed strong signs of antibody affinity maturation before differentiating into MBCs and ASCs. Thus, pre-existing human MBCs differentiate into ASCs in response to novel antigens, but the quality of the humoral and cellular anti-S response improved through the clonal selection and affinity maturation of naive precursors. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: SARS-CoV-2; affinity maturation; germinal center; humoral immunity; mRNA vaccination; pre-existing memory B cells; somatic hypermutation |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Spike Glycoprotein, Coronavirus) 0 (COVID-19 Vaccines) 0 (spike protein, SARS-CoV-2) 0 (Antibodies, Monoclonal) |
| تواريخ الأحداث: | Date Created: 20240821 Date Completed: 20240911 Latest Revision: 20240912 |
| رمز التحديث: | 20240913 |
| DOI: | 10.1016/j.immuni.2024.07.023 |
| PMID: | 39168129 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1097-4180 |
|---|---|
| DOI: | 10.1016/j.immuni.2024.07.023 |