دورية أكاديمية
أعرض في EDS

Fibroblast growth factor 2 promotes osteo/odontogenic differentiation in stem cells from the apical papilla by inhibiting PI3K/AKT pathway.

التفاصيل البيبلوغرافية
العنوان: Fibroblast growth factor 2 promotes osteo/odontogenic differentiation in stem cells from the apical papilla by inhibiting PI3K/AKT pathway.
المؤلفون: Wang Z; Hospital of Stomatology, Zunyi Medical University, Zunyi, 563000, Guizhou, China., Chen C; School of Stomatology, Southern Medical University, Guangzhou, 510000, Guangdong, China.; Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, 510000, China., Sun L; Hospital of Stomatology, Zunyi Medical University, Zunyi, 563000, Guizhou, China., He M; Hospital of Stomatology, Zunyi Medical University, Zunyi, 563000, Guizhou, China., Huang T; Hospital of Stomatology, Zunyi Medical University, Zunyi, 563000, Guizhou, China., Zheng J; Hospital of Stomatology, Zunyi Medical University, Zunyi, 563000, Guizhou, China., Wu J; Hospital of Stomatology, Zunyi Medical University, Zunyi, 563000, Guizhou, China. wujiayuan@zmu.edu.cn.
المصدر: Scientific reports [Sci Rep] 2024 Aug 21; Vol. 14 (1), pp. 19354. Date of Electronic Publication: 2024 Aug 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Fibroblast Growth Factor 2*/pharmacology , Fibroblast Growth Factor 2*/metabolism , Proto-Oncogene Proteins c-akt*/metabolism , Cell Differentiation*/drug effects , Osteogenesis*/drug effects , Signal Transduction*/drug effects , Dental Papilla*/cytology , Dental Papilla*/metabolism , Odontogenesis*/drug effects , Stem Cells*/metabolism , Stem Cells*/cytology , Stem Cells*/drug effects, Humans ; Phosphatidylinositol 3-Kinases/metabolism ; Cells, Cultured
مستخلص: Fibroblast growth factor 2 (FGF2) is a crucial factor in odontoblast differentiation and dentin matrix deposition, which facilitates pulpodentin repair and regeneration. Nevertheless, the specific biological function of FGF2 in odontoblastic differentiation remains unclear because it is controlled by complex signalling pathways. This study aimed to investigate the mechanism underlying the effect of FGF2 on osteo/odontogenic differentiation of stem cells from the apical papilla (SCAP). SCAP were pretreated with conditioned media containing FGF2 for 1 week, followed by culturing in induced differentiation medium for another week. RNA sequencing (RNA-seq) combined with quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to evaluate the pathways affected by FGF2 in SCAP. Osteo/odontogenic differentiation of SCAP was determined using Alizarin red S staining, alkaline phosphatase staining, RT-qPCR, and western blotting. Pretreatment with FGF2 for 1 week increased the osteo/odontogenic differentiation ability of SCAP. RNA-seq and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that phosphatidylinositol 3-kinase (PI3K)/AKT signalling is involved in the osteogenic function of FGF2. RT-qPCR results indicated that SCAP expressed FGF receptors, and western blotting showed that p-AKT was reduced in FGF2-pretreated SCAP. The activation of the PI3K/AKT pathway partially reversed the stimulatory effect of FGF2 on osteo/odontogenic differentiation of SCAP. Our findings suggest that pretreatment with FGF2 enhances the osteo/odontogenic differentiation ability of SCAP by inhibiting the PI3K/AKT pathway.
(© 2024. The Author(s).)
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معلومات مُعتمدة: gzwjkj2020-1-163 Guizhou Provincial Health Commission Science and Technology Fund Project; QKH- Foundation-ZK (2022) General 638 Science and Technology Plan Project of Guizhou Province; (2020)293 The Etiology and Prevention Team Project of Oral Infectious and Malignant Diseases in the Affiliated Stomatological Hospital of Zunyi Medical University
المشرفين على المادة: 103107-01-3 (Fibroblast Growth Factor 2)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
تواريخ الأحداث: Date Created: 20240821 Date Completed: 20240821 Latest Revision: 20240824
رمز التحديث: 20240826
مُعرف محوري في PubMed: PMC11339349
DOI: 10.1038/s41598-024-70123-0
PMID: 39169066
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-70123-0