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Engineering yeast for high-level production of β-farnesene from sole methanol.

التفاصيل البيبلوغرافية
العنوان: Engineering yeast for high-level production of β-farnesene from sole methanol.
المؤلفون: Li J; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China., Gao J; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China., Ye M; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China., Cai P; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China., Yu W; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China., Zhai X; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China., Zhou YJ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. Electronic address: zhouyongjin@dicp.ac.cn.
المصدر: Metabolic engineering [Metab Eng] 2024 Sep; Vol. 85, pp. 194-200. Date of Electronic Publication: 2024 Aug 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: Belgium NLM ID: 9815657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-7184 (Electronic) Linking ISSN: 10967176 NLM ISO Abbreviation: Metab Eng Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Brugge, Belgium ; Orlando, FL : Academic Press, c1999-
مواضيع طبية MeSH: Metabolic Engineering* , Methanol*/metabolism , Sesquiterpenes*/metabolism
مستخلص: Methanol, a rich one-carbon feedstock, can be massively produced from CO 2 by the liquid sunshine route, which is helpful to realize carbon neutrality. β-Farnesene is widely used in the production of polymers, surfactants, lubricants, and also serves as a suitable substitute for jet fuel. Constructing an efficient cell factory is a feasible approach for β-farnesene production through methanol biotransformation. Here, we extensively engineered the methylotrophic yeast Ogataea polymorpha for the efficient bio-production of β-farnesene using methanol as the sole carbon source. Our study demonstrated that sufficient supply of precursor acetyl-CoA and cofactor NADPH in an excellent yeast chassis had a 1.3-fold higher β-farnesene production than that of wild-type background strain. Further optimization of the mevalonate pathway and enhancement of acetyl-CoA supply led to a 7-fold increase in β-farnesene accumulation, achieving the highest reported sesquiterpenoids production (14.7 g/L with a yield of 46 mg/g methanol) from one-carbon feedstock under fed-batch fermentation in bioreactor. This study demonstrates the great potential of engineering O. polymorpha for high-level terpenoid production from methanol.
Competing Interests: Declaration of competing interest The authors have filed a patent (202410891936.1) for protecting the production of β-farnesene in O. polymorpha.
(Copyright © 2024 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Metabolic engineering; Methanol biotransformation; Ogataea polymorpha; β-farnesene
المشرفين على المادة: Y4S76JWI15 (Methanol)
0 (Sesquiterpenes)
18794-84-8 (beta-farnesene)
تواريخ الأحداث: Date Created: 20240824 Date Completed: 20240916 Latest Revision: 20240916
رمز التحديث: 20240917
DOI: 10.1016/j.ymben.2024.08.006
PMID: 39181436
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-7184
DOI:10.1016/j.ymben.2024.08.006