دورية أكاديمية
أعرض في EDS

Long-term passage impacts human dental pulp stem cell activities and cell response to drug addition in vitro .

التفاصيل البيبلوغرافية
العنوان: Long-term passage impacts human dental pulp stem cell activities and cell response to drug addition in vitro .
المؤلفون: Patntirapong S; Thammasat University Research Unit in Dental and Bone Substitute Biomaterials, Faculty of Dentistry, Thammasat University, Pathumthani, Thailand., Khankhow J; Faculty of Dentistry, Thammasat University, Pathumthani, Thailand., Julamorn S; Faculty of Dentistry, Thammasat University, Pathumthani, Thailand.
المصدر: PeerJ [PeerJ] 2024 Aug 23; Vol. 12, pp. e17913. Date of Electronic Publication: 2024 Aug 23 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Internet ISSN: 2167-8359 (Electronic) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Corte Madera, CA : PeerJ Inc.
مواضيع طبية MeSH: Dental Pulp*/cytology , Dental Pulp*/drug effects , Cell Proliferation*/drug effects , Alendronate*/pharmacology , Cell Adhesion*/drug effects, Humans ; Alkaline Phosphatase/metabolism ; Cells, Cultured ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/metabolism ; Mesenchymal Stem Cells/cytology ; Cell Culture Techniques/methods ; Stem Cells/drug effects ; Stem Cells/metabolism ; Stem Cells/cytology ; Cell Differentiation/drug effects
مستخلص: Background: Dental pulp stem cells (DPSCs) possess mesenchymal stem cell characteristics and have potential for cell-based therapy. Cell expansion is essential to achieve sufficient cell numbers. However, continuous cell replication causes cell aging in vitro , which usually accompanies and potentially affect DPSC characteristics and activities. Continuous passaging could alter susceptibility to external factors such as drug treatment. Therefore, this study sought to investigate potential outcome of in vitro passaging on DPSC morphology and activities in the absence or presence of external factor.
Methods: Human DPSCs were subcultured until reaching early passages (P5), extended passages (P10), and late passages (P15). Cells were evaluated and compared for cell and nuclear morphologies, cell adhesion, proliferative capacity, alkaline phosphatase (ALP) activity, and gene expressions in the absence or presence of external factor. Alendronate (ALN) drug treatment was used as an external factor.
Results: Continuous passaging of DPSCs gradually lost their normal spindle shape and increased in cell and nuclear sizes. DPSCs were vulnerable to ALN. The size and shape were altered, leading to morphological abnormality and inhomogeneity. Long-term culture and ALN interfered with cell adhesion. DPSCs were able to proliferate irrespective of cell passages but the rate of cell proliferation in late passages was slower. ALN at moderate dose inhibited cell growth. ALN caused reduction of ALP activity in early passage. In contrast, extended passage responded differently to ALN by increasing ALP activity. Late passage showed higher collagen but lower osteocalcin gene expressions compared with early passage in the presence of ALN.
Conclusion: An increase in passage number played critical role in cell morphology and activities as well as responses to the addition of an external factor. The effects of cell passage should be considered when used in basic science research and clinical applications.
Competing Interests: The authors declare there are no competing interests.
(©2024 Patntirapong et al.)
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فهرسة مساهمة: Keywords: Alendronate; Alkaline phosphatase activity; Cell morphology; Cell passage; Nuclear morphology; Proliferation
المشرفين على المادة: X1J18R4W8P (Alendronate)
EC 3.1.3.1 (Alkaline Phosphatase)
تواريخ الأحداث: Date Created: 20240828 Date Completed: 20240828 Latest Revision: 20240829
رمز التحديث: 20240830
مُعرف محوري في PubMed: PMC11348901
DOI: 10.7717/peerj.17913
PMID: 39193517
قاعدة البيانات: MEDLINE
الوصف
تدمد:2167-8359
DOI:10.7717/peerj.17913