دورية أكاديمية
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Molecular Characteristics of the Malate Dehydrogenase (MDH) Gene Family in Spirometra mansoni (Cestoda: Diphyllobothriidea).

التفاصيل البيبلوغرافية
العنوان: Molecular Characteristics of the Malate Dehydrogenase (MDH) Gene Family in Spirometra mansoni (Cestoda: Diphyllobothriidea).
المؤلفون: Wang R; Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China., Hao J; Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China., Cao C; Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China., Li J; Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China., Zhang X; Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Aug 13; Vol. 25 (16). Date of Electronic Publication: 2024 Aug 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Phylogeny* , Spirometra*/genetics , Spirometra*/enzymology , Malate Dehydrogenase*/genetics , Malate Dehydrogenase*/metabolism , Malate Dehydrogenase*/chemistry, Animals ; Helminth Proteins/genetics ; Helminth Proteins/chemistry ; Helminth Proteins/metabolism ; Multigene Family ; Amino Acid Sequence
مستخلص: The plerocercoid larva of Spirometra mansoni can cause a parasitic zoonosis-sparganosis. Malate dehydrogenase (MDH) plays a very important role in the life activities of parasites. However, little is known about the MDH family in S. mansoni . We identified eight new MDH members in S. mansoni in this study. Clustering analysis divided Sm MDHs into two groups and revealed patterns similar to the conserved motif organization. RT-qPCR suggested that five MDHs were highly expressed in the mature proglottid and that three MDHs were highly expressed in the gravid proglottid. Phylogenetic analysis revealed that Sm MDHs contain both conserved family members and members in the process of further diversification. r Sm MDH has an NAD binding domain, a dimer interface and a substrate binding domain. Natural Sm MDH was immunolocalized in the tissues and follicles around the uterus in the mature or gravid proglottid and eggshells. The maximum forward and reverse reaction activities of r Sm MDH were observed at pH 8.5 and 9.0, respectively. The optimum temperature for enzyme activity was 37 °C in the forward reaction and 40 °C in the reverse reaction. These results lay the foundation for studying the molecular functions and mechanisms of MDHs in S. mansoni and related taxa.
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معلومات مُعتمدة: 24ZX003 Fundamental Research Project of key scientific research in Henan Province
فهرسة مساهمة: Keywords: Spirometra mansoni; gene expression; malate dehydrogenase; molecular characterization; tapeworm
المشرفين على المادة: EC 1.1.1.37 (Malate Dehydrogenase)
0 (Helminth Proteins)
تواريخ الأحداث: Date Created: 20240829 Date Completed: 20240829 Latest Revision: 20240903
رمز التحديث: 20240904
مُعرف محوري في PubMed: PMC11354392
DOI: 10.3390/ijms25168802
PMID: 39201488
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25168802