دورية أكاديمية
أعرض في EDS

Identification and Characterization of a Novel B Cell Epitope of ASFV Virulence Protein B125R Monoclonal Antibody.

التفاصيل البيبلوغرافية
العنوان: Identification and Characterization of a Novel B Cell Epitope of ASFV Virulence Protein B125R Monoclonal Antibody.
المؤلفون: Zhao Y; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Ren H; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Lin Z; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Shi S; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Zhang B; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Zhang Y; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Han S; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., He WR; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Wan B; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Hu M; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China., Zhang GP; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.; Longhu Laboratory, Zhengzhou 450046, China.
المصدر: Viruses [Viruses] 2024 Aug 05; Vol. 16 (8). Date of Electronic Publication: 2024 Aug 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Epitopes, B-Lymphocyte*/immunology , Epitopes, B-Lymphocyte*/genetics , African Swine Fever Virus*/immunology , African Swine Fever Virus*/genetics , Antibodies, Monoclonal*/immunology , Antibodies, Viral*/immunology, Animals ; Mice ; Mice, Inbred BALB C ; Swine ; African Swine Fever/immunology ; African Swine Fever/virology ; Virulence ; Epitope Mapping ; Female
مستخلص: The African swine fever virus (ASFV) is an ancient, structurally complex, double-stranded DNA virus that causes African swine fever. Since its discovery in Kenya and Africa in 1921, no effective vaccine or antiviral strategy has been developed. Therefore, the selection of more suitable vaccines or antiviral targets is the top priority to solve the African swine fever virus problem. B125R , one of the virulence genes of ASFV, encodes a non-structural protein (pB125R), which is important in ASFV infection. However, the epitope of pB125R is not well characterized at present. We observed that pB125R is specifically recognized by inactivated ASFV-positive sera, suggesting that it has the potential to act as a protective antigen against ASFV infection. Elucidation of the antigenic epitope within pB125R could facilitate the development of an epitope-based vaccine targeting ASFV. In this study, two strains of monoclonal antibodies (mAbs) against pB125R were produced by using the B cell hybridoma technique, named 9G11 and 15A9. The antigenic epitope recognized by mAb 9G11 was precisely located by using a series of truncated ASFV pB125R. The 52 DPLASQRDIYY 62 (epitope on ASFV pB125R) was the smallest epitope recognized by mAb 9G11 and this epitope was highly conserved among different strains. The key amino acid sites were identified as D52, Q57, R58, and Y62 by the single-point mutation of 11 amino acids of the epitope by alanine scanning. In addition, the immunological effects of the epitope (pB125R-DY) against 9G11 were evaluated in mice, and the results showed that both full-length pB125R and the epitope pB125R-DY could induce effective humoral and cellular immune responses in mice. The mAbs obtained in this study reacted with the eukaryotic-expressed antigen proteins and the PAM cell samples infected with ASFV, indicating that the mAb can be used as a good tool for the detection of ASFV antigen infection. The B cell epitopes identified in this study provide a fundamental basis for the research and development of epitope-based vaccines against ASFV.
References: J Gen Virol. 2008 Feb;89(Pt 2):397-408. (PMID: 18198370)
Emerg Infect Dis. 2018 Nov;24(11):2131-2133. (PMID: 30141772)
J Nanobiotechnology. 2023 Nov 14;21(1):424. (PMID: 37964304)
J Virol. 2020 Mar 17;94(7):. (PMID: 31969432)
J Virol. 2022 Mar 9;96(5):e0193921. (PMID: 35019713)
Science. 2019 Nov 1;366(6465):640-644. (PMID: 31624094)
Viruses. 2022 Aug 30;14(9):. (PMID: 36146726)
Vaccines (Basel). 2019 Jun 25;7(2):. (PMID: 31242632)
Transbound Emerg Dis. 2017 Oct;64(5):1479-1492. (PMID: 27390151)
Clin Vaccine Immunol. 2009 Jul;16(7):1012-20. (PMID: 19420186)
Front Vet Sci. 2018 Mar 05;5:31. (PMID: 29556501)
Viruses. 2022 Dec 31;15(1):. (PMID: 36680174)
Virus Res. 2020 Apr 2;279:197871. (PMID: 32004574)
J Virol. 2006 Dec;80(24):12260-70. (PMID: 17035321)
Transbound Emerg Dis. 2017 Dec;64(6):1665-1668. (PMID: 28921873)
Virus Res. 2024 May;343:199343. (PMID: 38423214)
Transbound Emerg Dis. 2020 Mar;67(2):473-475. (PMID: 31955520)
J Vet Res. 2020 Jun 16;64(2):207-213. (PMID: 32587906)
J Virol. 2022 Jul 27;96(14):e0054522. (PMID: 35862691)
Annu Rev Anim Biosci. 2020 Feb 15;8:221-246. (PMID: 31743062)
Vet Microbiol. 2023 Aug;283:109776. (PMID: 37270924)
Front Microbiol. 2023 Feb 03;14:1126794. (PMID: 36819042)
معلومات مُعتمدة: 32272987 Bo Wan; 32102655 Wenrui He; 32373005 Shichong Han; 221100110600 Gaiping zhang; 2022YFD1801300 Wenrui He; 222102110453 Junqing Guo
فهرسة مساهمة: Keywords: African swine fever virus; epitope identification; monoclonal antibody; pB125R
المشرفين على المادة: 0 (Epitopes, B-Lymphocyte)
0 (Antibodies, Monoclonal)
0 (Antibodies, Viral)
تواريخ الأحداث: Date Created: 20240829 Date Completed: 20240829 Latest Revision: 20240903
رمز التحديث: 20240903
مُعرف محوري في PubMed: PMC11359698
DOI: 10.3390/v16081257
PMID: 39205231
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v16081257