دورية أكاديمية
Impact of Cellular Crowding on Protein Structural Dynamics Investigated by EPR Spectroscopy.
| العنوان: | Impact of Cellular Crowding on Protein Structural Dynamics Investigated by EPR Spectroscopy. |
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| المؤلفون: | Pierro A; Department of Chemistry, Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany., Bonucci A; Aix Marseille University, CNRS, Bioénergétique et Ingénierie des Protéines (BIP), IMM, IM2B, Marseille, France., Magalon A; Aix Marseille University, CNRS, Laboratoire de Chimie Bactérienne (LCB), IMM, IM2B, Marseille, France., Belle V; Aix Marseille University, CNRS, Bioénergétique et Ingénierie des Protéines (BIP), IMM, IM2B, Marseille, France., Mileo E; Aix Marseille University, CNRS, Bioénergétique et Ingénierie des Protéines (BIP), IMM, IM2B, Marseille, France. |
| المصدر: | Chemical reviews [Chem Rev] 2024 Sep 11; Vol. 124 (17), pp. 9873-9898. Date of Electronic Publication: 2024 Aug 30. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 2985134R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6890 (Electronic) Linking ISSN: 00092665 NLM ISO Abbreviation: Chem Rev Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Chemical Society Original Publication: Easton, Pa. [etc.] American Chemical Society. |
| مواضيع طبية MeSH: | Proteins*/chemistry , Proteins*/metabolism , Spin Labels*, Electron Spin Resonance Spectroscopy/methods ; Humans ; Protein Conformation ; Animals |
| مستخلص: | The study of how the intracellular medium influences protein structural dynamics and protein-protein interactions is a captivating area of research for scientists aiming to comprehend biomolecules in their native environment. As the cellular environment can hardly be reproduced in vitro , direct investigation of biomolecules within cells has attracted growing interest in the past two decades. Among magnetic resonances, site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy (SDSL-EPR) has emerged as a powerful tool for studying the structural properties of biomolecules directly in cells. Since the first in-cell EPR experiment was reported in 2010, substantial progress has been made, and this Review provides a detailed overview of the developments and applications of this spectroscopic technique. The strategies available for preparing a cellular sample and the EPR methods that can be applied to cells will be discussed. The array of spin labels available, along with their strengths and weaknesses in cellular contexts, will also be described. Several examples will illustrate how in-cell EPR can be applied to different biological systems and how the cellular environment affects the structural and dynamic properties of different proteins. Lastly, the Review will focus on the future developments expected to expand the capabilities of this promising technique. |
| المشرفين على المادة: | 0 (Proteins) 0 (Spin Labels) |
| تواريخ الأحداث: | Date Created: 20240830 Date Completed: 20240911 Latest Revision: 20240911 |
| رمز التحديث: | 20240911 |
| DOI: | 10.1021/acs.chemrev.3c00951 |
| PMID: | 39213496 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6890 |
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| DOI: | 10.1021/acs.chemrev.3c00951 |