دورية أكاديمية
أعرض في EDS

Overexpression of Two Odorant Binding Proteins Confers Chlorpyrifos Resistance in the Green Peach Aphid Myzus persicae .

التفاصيل البيبلوغرافية
العنوان: Overexpression of Two Odorant Binding Proteins Confers Chlorpyrifos Resistance in the Green Peach Aphid Myzus persicae .
المؤلفون: Xiao X; Department of Entomology, China Agricultural University, Beijing 100193, China., Yin XH; Department of Entomology, China Agricultural University, Beijing 100193, China., Hu SY; Department of Entomology, China Agricultural University, Beijing 100193, China., Miao HN; Department of Entomology, China Agricultural University, Beijing 100193, China., Wang Z; Department of Entomology, China Agricultural University, Beijing 100193, China.; Sanya Institute of China Agricultural University, Sanya 572024, China., Li H; Department of Entomology, China Agricultural University, Beijing 100193, China., Zhang YJ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Liang P; Department of Entomology, China Agricultural University, Beijing 100193, China., Gu SH; Department of Entomology, China Agricultural University, Beijing 100193, China.; Sanya Institute of China Agricultural University, Sanya 572024, China.
المصدر: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Sep 11; Vol. 72 (36), pp. 20101-20113. Date of Electronic Publication: 2024 Sep 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Aphids*/genetics , Aphids*/drug effects , Aphids*/metabolism , Chlorpyrifos*/metabolism , Chlorpyrifos*/pharmacology , Receptors, Odorant*/genetics , Receptors, Odorant*/metabolism , Receptors, Odorant*/chemistry , Insecticide Resistance*/genetics , Insect Proteins*/genetics , Insect Proteins*/metabolism , Insect Proteins*/chemistry , Insecticides*/pharmacology , Insecticides*/metabolism, Animals ; Prunus persica/genetics ; Prunus persica/parasitology ; Prunus persica/metabolism ; Prunus persica/chemistry
مستخلص: The green peach aphid, Myzus persicae , is a worldwide agricultural pest. Chlorpyrifos has been widely used to control M. persicae for decades, thus leading to a high resistance to chlorpyrifos. Recent studies have found that insect odorant binding proteins (OBPs) play essential roles in insecticide resistance. However, the potential resistance mechanism underlying the cross-link between aphid OBPs and chlorpyrifos remains unclear. In this study, two OBPs (MperOBP3 and MperOBP7) were found overexpressed in M. persicae chlorpyrifos-resistant strains (CRR) compared to chlorpyrifos-sensitive strains (CSS); furthermore, chlorpyrifos can significantly induce the expression of both OBPs. An in vitro binding assay indicated that both OBPs strongly bind with chlorpyrifos; an in vivo RNAi and toxicity bioassay confirmed silencing either of the two OBPs can increase the susceptibility of aphids to chlorpyrifos, suggesting that overexpression of MperOBP3 and MperOBP7 contributes to the development of resistance of M. persicae to chlorpyrifos. Our findings provide novel insights into insect OBPs-mediated resistance mechanisms.
فهرسة مساهمة: Keywords: Myzus persicae; OBPs; binding assay; chlorpyrifos; insecticide resistance; molecular docking
المشرفين على المادة: JCS58I644W (Chlorpyrifos)
0 (odorant-binding protein)
0 (Receptors, Odorant)
0 (Insect Proteins)
0 (Insecticides)
تواريخ الأحداث: Date Created: 20240902 Date Completed: 20240911 Latest Revision: 20240911
رمز التحديث: 20240911
DOI: 10.1021/acs.jafc.4c05026
PMID: 39223077
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5118
DOI:10.1021/acs.jafc.4c05026