دورية أكاديمية
أعرض في EDS

Detection and molecular characterization of major enteric pathogens in calves in central Ethiopia.

التفاصيل البيبلوغرافية
العنوان: Detection and molecular characterization of major enteric pathogens in calves in central Ethiopia.
المؤلفون: Bergholm J; Department of Animal Biosciences, Swedish University of Agricultural Sciences, Uppsala, Sweden. julia.bergholm@slu.se., Tessema TS; Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia., Blomström AL; Department of Animal Biosciences, Swedish University of Agricultural Sciences, Uppsala, Sweden., Berg M; Department of Animal Biosciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
المصدر: BMC veterinary research [BMC Vet Res] 2024 Sep 04; Vol. 20 (1), pp. 389. Date of Electronic Publication: 2024 Sep 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Diarrhea*/veterinary , Diarrhea*/virology , Diarrhea*/microbiology , Diarrhea*/parasitology , Cattle Diseases*/virology , Cattle Diseases*/epidemiology , Cattle Diseases*/microbiology , Cattle Diseases*/parasitology , Feces*/virology , Feces*/parasitology , Feces*/microbiology , Rotavirus*/genetics , Rotavirus*/isolation & purification , Rotavirus*/classification , Cryptosporidium*/isolation & purification , Cryptosporidium*/genetics , Cryptosporidium*/classification , Coronavirus, Bovine*/genetics , Coronavirus, Bovine*/isolation & purification, Animals ; Cattle ; Ethiopia/epidemiology ; Enterotoxigenic Escherichia coli/isolation & purification ; Enterotoxigenic Escherichia coli/genetics ; Genotype ; Cryptosporidiosis/epidemiology ; Rotavirus Infections/veterinary ; Rotavirus Infections/epidemiology ; Rotavirus Infections/virology ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Coronavirus Infections/epidemiology ; Real-Time Polymerase Chain Reaction/veterinary ; Escherichia coli Infections/veterinary ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/microbiology
مستخلص: Background: Calf diarrhea is a major cause of morbidity and mortality in the livestock sector worldwide and it can be caused by multiple infectious agents. In Ethiopia, cattle are the most economically important species within the livestock sector, but at the same time the young animals suffer from high rates of morbidity and mortality due to calf diarrhea. However, studies including both screening and molecular characterization of bovine enteric pathogens are lacking. Therefore, we aimed to both detect and molecularly characterize four of the major enteric pathogens in calf diarrhea, Enterotoxigenic Escherichia coli (E. coli K99 +), Cryptosporidium spp., rotavirus A (RVA), and bovine coronavirus (BCoV) in calves from central Ethiopia. Diarrheic and non-diarrheic calves were included in the study and fecal samples were analyzed with antigen-ELISA and quantitative real-time PCR (qPCR). Positive samples were further characterized by genotyping PCRs.
Results: All four pathogens were detected in both diarrheic and non-diarrheic calves using qPCR and further characterization showed the presence of three Cryptosporidium species, C. andersoni, C. bovis and C. ryanae. Furthermore, genotyping of RVA-positive samples found a common bovine genotype G10P[11], as well as a more unusual G-type, G24. To our knowledge this is the first detection of the G24 RVA genotype in Ethiopia as well as in Africa. Lastly, investigation of the spike gene revealed two distinct BCoV strains, one classical BCoV strain and one bovine-like CoV strain.
Conclusions: Our results show that Cryptosporidium spp., E. coli K99 + , RVA and BCoV circulate in calves from central Ethiopia. Furthermore, our findings of the rare RVA G-type G24 and a bovine-like CoV demonstrates the importance of genetic characterization.
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Cryptosporidium; E. coli K99 + ; Bovine coronavirus; Calf diarrhea; Ethiopia; Rotavirus A
تواريخ الأحداث: Date Created: 20240903 Date Completed: 20240904 Latest Revision: 20240906
رمز التحديث: 20240906
مُعرف محوري في PubMed: PMC11373192
DOI: 10.1186/s12917-024-04258-7
PMID: 39227796
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-024-04258-7