دورية أكاديمية
Profiling migration of human monocytes in response to chemotactic and barotactic guidance cues.
| العنوان: | Profiling migration of human monocytes in response to chemotactic and barotactic guidance cues. |
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| المؤلفون: | Hall CK; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA., Barr OM; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA., Delamare A; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA., Burkholder A; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA., Tsai A; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA., Tian Y; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA., Felix E Ellett; Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Charlestown, MA, USA; Harvard Medical School, Boston, MA, USA., Li BM; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA., Tanzi RE; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA; Harvard Medical School, Boston, MA, USA. Electronic address: rtanzi@mgh.harvard.edu., Jorfi M; Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA; Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Charlestown, MA, USA; Harvard Medical School, Boston, MA, USA. Electronic address: mjorfi@mgh.harvard.edu. |
| المصدر: | Cell reports methods [Cell Rep Methods] 2024 Sep 16; Vol. 4 (9), pp. 100846. Date of Electronic Publication: 2024 Sep 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc Country of Publication: United States NLM ID: 9918227360606676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2667-2375 (Electronic) Linking ISSN: 26672375 NLM ISO Abbreviation: Cell Rep Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [New York] : Elsevier Inc., [2021]- |
| مواضيع طبية MeSH: | Monocytes*/immunology , Monocytes*/metabolism , Monocytes*/drug effects , Cell Movement*/drug effects , Chemotaxis*/drug effects, Humans ; Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology ; Chemokine CCL2/metabolism ; Interferon-gamma/pharmacology ; Interferon-gamma/metabolism ; Receptors, CCR2/metabolism ; Adult |
| مستخلص: | Monocytes are critical to innate immunity, participating in chemotaxis during tissue injury, infection, and inflammatory conditions. However, the migration dynamics of human monocytes under different guidance cues are not well characterized. Here, we developed a microfluidic device to profile the migration characteristics of human monocytes under chemotactic and barotactic guidance cues while also assessing the effects of age and cytokine stimulation. Human monocytes preferentially migrated toward the CCL2 gradient through confined microchannels, regardless of donor age and migration pathway. Stimulation with interferon (IFN)-γ, but not granulocyte-macrophage colony-stimulating factor (GM-CSF), disrupted monocyte navigation through complex paths and decreased monocyte CCL2 chemotaxis, velocity, and CCR2 expression. Additionally, monocytes exhibited a bias toward low-hydraulic-resistance pathways in asymmetric environments, which remained consistent across donor ages, cytokine stimulation, and chemoattractants. This microfluidic system provides insights into the unique migratory behaviors of human monocytes and is a valuable tool for studying peripheral immune cell migration in health and disease. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CP: Biotechnology; CP: Immunology; cell migration; chemotaxis; microfluidic; monocytes; peripheral immune cells |
| المشرفين على المادة: | 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor) 0 (Chemokine CCL2) 82115-62-6 (Interferon-gamma) 0 (Receptors, CCR2) 0 (CCR2 protein, human) 0 (CCL2 protein, human) |
| تواريخ الأحداث: | Date Created: 20240906 Date Completed: 20240917 Latest Revision: 20240917 |
| رمز التحديث: | 20240918 |
| DOI: | 10.1016/j.crmeth.2024.100846 |
| PMID: | 39241776 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2667-2375 |
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| DOI: | 10.1016/j.crmeth.2024.100846 |