دورية أكاديمية
Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence.
| العنوان: | Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence. |
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| المؤلفون: | Martinez-Ordoñez A; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: amo4002@med.cornell.edu., Kinoshita H; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA., Cid-Diaz T; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA., Duran A; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA., Osrodek M; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA., Diaz-Meco MT; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA., Moscat J; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: jom4010@med.cornell.edu. |
| المصدر: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103303. Date of Electronic Publication: 2024 Sep 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Epithelial Cells*/cytology , Epithelial Cells*/metabolism , Cell Lineage* , Fluorescent Antibody Technique*/methods , Intestinal Mucosa*/cytology , Intestinal Mucosa*/metabolism, Animals ; Mice ; Colon/cytology ; Colon/metabolism ; Intestine, Small/cytology ; Intestine, Small/metabolism |
| مستخلص: | Applying Opal multiplex immunofluorescence (OMI) to characterize intestinal tissues of genetically engineered mouse models provides an excellent tool for studying complex processes. However, detecting appropriate signals from multiple target molecules is challenging. Here, we present a protocol to characterize mouse intestinal epithelial cell lineage using OMI. We describe steps for processing small intestine and colonic mouse tissues and designing and optimizing panels for OMI in mouse intestinal tissues. We then detail procedures for performing a quantitative evaluation of acquired images. For complete details on the use and execution of this protocol, please refer to Kinoshita et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| References: | Immunity. 2018 Dec 18;49(6):1132-1147.e7. (PMID: 30552022) Cancer Cell. 2023 Feb 13;41(2):252-271.e9. (PMID: 36525970) Dev Cell. 2024 Aug 5;59(15):1972-1987.e8. (PMID: 38815584) |
| فهرسة مساهمة: | Keywords: Cancer; In Situ Hybridization; Microscopy; Model Organisms; Molecular Biology; Molecular/Chemical Probes; Stem Cells |
| تواريخ الأحداث: | Date Created: 20240912 Date Completed: 20240926 Latest Revision: 20240927 |
| رمز التحديث: | 20240927 |
| مُعرف محوري في PubMed: | PMC11422768 |
| DOI: | 10.1016/j.xpro.2024.103303 |
| PMID: | 39264806 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2024.103303 |