دورية أكاديمية
أعرض في EDS

Turning the needle into the haystack: Culture-independent amplification of complex microbial genomes directly from their native environment.

التفاصيل البيبلوغرافية
العنوان: Turning the needle into the haystack: Culture-independent amplification of complex microbial genomes directly from their native environment.
المؤلفون: Pilling OA; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America., Sundararaman SA; Department of Pediatrics, Children's Hospital of Philadelphia, The Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America., Brisson D; Department of Biology, School of Arts & Sciences, University of Pennsylvania, Pennsylvania, United States of America., Beiting DP; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
المصدر: PLoS pathogens [PLoS Pathog] 2024 Sep 12; Vol. 20 (9), pp. e1012418. Date of Electronic Publication: 2024 Sep 12 (Print Publication: 2024).
نوع المنشور: Journal Article; Review
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: High-Throughput Nucleotide Sequencing*/methods, Humans ; Genome, Bacterial ; Nucleic Acid Amplification Techniques/methods ; Bacteria/genetics ; Genome, Microbial ; Whole Genome Sequencing/methods
مستخلص: High-throughput sequencing (HTS) has revolutionized microbiology, but many microbes exist at low abundance in their natural environment and/or are difficult, if not impossible, to culture in the laboratory. This makes it challenging to use HTS to study the genomes of many important microbes and pathogens. In this review, we discuss the development and application of selective whole genome amplification (SWGA) to allow whole or partial genomes to be sequenced for low abundance microbes directly from complex biological samples. We highlight ways in which genomic data generated by SWGA have been used to elucidate the population dynamics of important human pathogens and monitor development of antimicrobial resistance and the emergence of potential outbreaks. We also describe the limitations of this method and propose some potential innovations that could be used to improve the quality of SWGA and lower the barriers to using this method across a wider range of infectious pathogens.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Pilling et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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تواريخ الأحداث: Date Created: 20240912 Date Completed: 20240912 Latest Revision: 20240914
رمز التحديث: 20240914
مُعرف محوري في PubMed: PMC11392400
DOI: 10.1371/journal.ppat.1012418
PMID: 39264872
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7374
DOI:10.1371/journal.ppat.1012418