دورية أكاديمية
Impact of sample refrigeration and freezing on the bacteriological counts of different bedding materials for dairy cows.
| العنوان: | Impact of sample refrigeration and freezing on the bacteriological counts of different bedding materials for dairy cows. |
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| المؤلفون: | Fusar Poli S; Department of Veterinary Medicine and Animal Sciences, University of Milan, Lodi, Italy., Monistero V; Department of Veterinary Medicine and Animal Sciences, University of Milan, Lodi, Italy., Pollera C; Department of Veterinary Medicine and Animal Sciences, University of Milan, Lodi, Italy.; Laboratory of Animal Infectious Diseases (MiLAB), University of Milan, Lodi, Italy., Freu G; Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, Brazil., Bronzo V; Department of Veterinary Medicine and Animal Sciences, University of Milan, Lodi, Italy.; Laboratory of Animal Infectious Diseases (MiLAB), University of Milan, Lodi, Italy., Piccinini R; Department of Veterinary Medicine and Animal Sciences, University of Milan, Lodi, Italy.; Laboratory of Animal Infectious Diseases (MiLAB), University of Milan, Lodi, Italy., Nocetti M; Consorzio del Formaggio Parmigiano Reggiano, Reggio Emilia, Italy., Sala G; Department of Veterinary Sciences, University of Pisa, Pisa, Italy., Veiga Dos Santos M; Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, Brazil., Moroni P; Department of Veterinary Medicine and Animal Sciences, University of Milan, Lodi, Italy.; Laboratory of Animal Infectious Diseases (MiLAB), University of Milan, Lodi, Italy.; Animal Health Diagnostic Center, Quality Milk Production Services (QMPS), Cornell University, Ithaca, NY, USA., Addis MF; Department of Veterinary Medicine and Animal Sciences, University of Milan, Lodi, Italy. filippa.addis@unimi.it.; Laboratory of Animal Infectious Diseases (MiLAB), University of Milan, Lodi, Italy. filippa.addis@unimi.it. |
| المصدر: | BMC veterinary research [BMC Vet Res] 2024 Sep 12; Vol. 20 (1), pp. 410. Date of Electronic Publication: 2024 Sep 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, 2005- |
| مواضيع طبية MeSH: | Refrigeration* , Freezing* , Manure*/microbiology , Dairying* , Bacterial Load*, Animals ; Cattle ; Female ; Housing, Animal ; Bacteria/isolation & purification ; Bacteria/classification |
| مستخلص: | Background: Different organic and inorganic bedding materials can be used in dairy farms. Among organic materials, there is an increasing interest in alternative substrates based on recycled manure solids (RMS). Microbiological analyses are crucial to monitor the microbial load and evaluate the presence of pathogens impacting animal welfare and health. However, logistic factors may hamper the possibility of immediately sending fresh samples to the laboratory, requiring storage in cooled conditions before analysis. Methods: We assessed the impact of sample refrigeration and freezing of different organic and inorganic bedding substrates including separated raw manure solids (SRMS), anaerobically digested manure solids (ADMS), and new sand (NS), on the total bacterial count (TBC) and on different microbial classes. Results: The TBC was higher in fresh NS and ADMS than in refrigerated and frozen samples of the same substrates; in addition, the TBC of ADMS was higher in refrigerated than frozen samples. The TBC of SRMS did not change significantly with refrigeration and freezing. Freezing reduced the total Gram-negative bacterial count more than refrigeration in all substrates. In fresh NS, Gram-negatives were higher than in both refrigerated and frozen NS. Escherichia coli counts were significantly lower in frozen than in refrigerated SRMS. However, both refrigeration and freezing of ADMS resulted in no E. coli growth. The coliform counts were also lower in frozen than refrigerated NS and SRMS. Frozen NS and ADMS showed lower counts compared to refrigeration for Gram-negative bacteria other than E. coli and coliforms. On the other hand, cold storage did not significantly impact the streptococci and streptococcus-like organisms (SSLO) count of all evaluated bedding substrates. Conclusion: Refrigeration and freezing affect the bacteriological results of bedding substrates, with freezing generally leading to lower counts than refrigeration. Whenever possible, preference should be given to analyzing fresh bedding samples, however, when necessary, refrigeration would be recommended over freezing, while acknowledging that the measured bacterial load might underestimate the actual microbial content. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | Piano Operativo Nazionale, Decreto Ministeriale 1061 (10.08.21); Azione IV.5 - Dottorati su tematiche Green Ministero dell'Università e della Ricerca; Finance Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| فهرسة مساهمة: | Keywords: Anaerobically digested manure solids; Bedding bacteriology; New sand; Separated raw manure solids |
| المشرفين على المادة: | 0 (Manure) |
| تواريخ الأحداث: | Date Created: 20240912 Date Completed: 20240913 Latest Revision: 20240915 |
| رمز التحديث: | 20240915 |
| مُعرف محوري في PubMed: | PMC11391596 |
| DOI: | 10.1186/s12917-024-04247-w |
| PMID: | 39267023 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1746-6148 |
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| DOI: | 10.1186/s12917-024-04247-w |