دورية أكاديمية
أعرض في EDS

Exosomal transcript cargo and functional correlation with HNSCC patients' survival.

التفاصيل البيبلوغرافية
العنوان: Exosomal transcript cargo and functional correlation with HNSCC patients' survival.
المؤلفون: Yadav J; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India., Chaudhary A; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India., Tripathi T; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India., Janjua D; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India., Joshi U; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India., Aggarwal N; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India., Chhokar A; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India.; Department of Zoology, Deshbandhu College, University of Delhi, Delhi, India., Keshavam CC; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India., Senrung A; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India.; Department of Zoology, Daulat Ram College, University of Delhi, Delhi, India., Bharti AC; Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India. alokchandrab@yahoo.com.
المصدر: BMC cancer [BMC Cancer] 2024 Sep 13; Vol. 24 (1), pp. 1144. Date of Electronic Publication: 2024 Sep 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967800 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2407 (Electronic) Linking ISSN: 14712407 NLM ISO Abbreviation: BMC Cancer Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Exosomes*/metabolism , Exosomes*/genetics , Squamous Cell Carcinoma of Head and Neck*/genetics , Squamous Cell Carcinoma of Head and Neck*/virology , Squamous Cell Carcinoma of Head and Neck*/mortality , Squamous Cell Carcinoma of Head and Neck*/pathology , Squamous Cell Carcinoma of Head and Neck*/metabolism , Head and Neck Neoplasms*/genetics , Head and Neck Neoplasms*/mortality , Head and Neck Neoplasms*/pathology , Head and Neck Neoplasms*/virology , Head and Neck Neoplasms*/metabolism, Humans ; Male ; Female ; Gene Expression Regulation, Neoplastic ; Gene Expression Profiling ; Papillomavirus Infections/virology ; Papillomavirus Infections/complications ; Papillomavirus Infections/genetics ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Middle Aged ; Cell Line, Tumor ; Transcriptome ; Prognosis ; Aged
مستخلص: Background: HPV status in a subset of HNSCC is linked with distinct treatment outcomes. Present investigation aims to elucidate the distinct clinicopathological features of HPV-positive and HPV-negative HNSCC and investigate their association with the HNSCC patient survival.
Materials and Methods: The total RNA of exosomes from HPV-positive (93VU147T) and HPV-negative (OCT-1) HNSCC cells was isolated, and the transcripts were estimated using Illumina HiSeq X. The expression of altered transcripts and their clinical relevance were further analyzed using publicly available cancer transcriptome data from The Cancer Genome Atlas (TCGA).
Results: Transcriptomic analyses identified 3785 differentially exported transcripts (DETs) in HPV-positive exosomes compared to HPV-negative exosomes. DETs that regulate the protein machinery, cellular redox potential, and various neurological disorder-related pathways were over-represented in HPV-positive exosomes. TCGA database revealed the clinical relevance of altered transcripts. Among commonly exported abundant transcripts, SGK1 and MAD1L1 showed high expression, which has been correlated with poor survival in HNSCC patients. In the top 20 DETs of HPV-negative exosomes, high expression of FADS3, SGK3, and TESK2 correlated with poor survival of the HNSCC patients in the TCGA database.
Conclusion: Overall, our study demonstrates that HPV-positive and HPV-negative cells' exosomes carried differential transcripts cargo that may be related to pathways associated with neurological disorders. Additionally, the altered transcripts identified have clinical relevance, correlating with patient survival in HNSCC, thereby highlighting their potential as biomarkers and as therapeutic targets.
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Differentially exported transcripts (DETs); Exosomes; Head and neck cancers (HNCs); Human papillomavirus (HPV); Illumina HiSeq; The Cancer Genome Atlas (TCGA)
المشرفين على المادة: 0 (Biomarkers, Tumor)
تواريخ الأحداث: Date Created: 20240913 Date Completed: 20240914 Latest Revision: 20240916
رمز التحديث: 20240916
مُعرف محوري في PubMed: PMC11395213
DOI: 10.1186/s12885-024-12759-9
PMID: 39272022
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2407
DOI:10.1186/s12885-024-12759-9