دورية أكاديمية
أعرض في EDS

Microbial synthesis of sedoheptulose from glucose by metabolically engineered Corynebacterium glutamicum.

التفاصيل البيبلوغرافية
العنوان: Microbial synthesis of sedoheptulose from glucose by metabolically engineered Corynebacterium glutamicum.
المؤلفون: Liu Y; Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China., Dong Q; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Song W; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China., Pei W; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China., Zeng Y; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China., Wang M; Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China., Sun Y; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Ma Y; Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. ma_yh@tib.cas.cn.; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. ma_yh@tib.cas.cn., Yang J; National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. yang_jg1@tib.cas.cn.
المصدر: Microbial cell factories [Microb Cell Fact] 2024 Sep 14; Vol. 23 (1), pp. 251. Date of Electronic Publication: 2024 Sep 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101139812 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2859 (Electronic) Linking ISSN: 14752859 NLM ISO Abbreviation: Microb Cell Fact Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2002-
مواضيع طبية MeSH: Corynebacterium glutamicum*/metabolism , Corynebacterium glutamicum*/genetics , Corynebacterium glutamicum*/enzymology , Metabolic Engineering*/methods , Heptoses*/biosynthesis , Heptoses*/metabolism , Glucose*/metabolism, Pentose Phosphate Pathway ; Fermentation
مستخلص: Background: Seven-carbon sugars, which rarely exist in nature, are the key constitutional unit of septacidin and hygromycin B in bacteria. These sugars exhibit a potential therapeutic effect for hypoglycaemia and cancer and serve as building blocks for the synthesis of C-glycosides and novel antibiotics. However, chemical and enzymatic approaches for the synthesis of seven-carbon sugars have faced challenges, such as complex reaction steps, low overall yields and high-cost feedstock, limiting their industrial-scale production.
Results: In this work, we propose a strain engineering approach for synthesising sedoheptulose using glucose as sole feedstock. The gene pfkA encoding 6-phosphofructokinase in Corynebacterium glutamicum was inactivated to direct the carbon flux towards the pentose phosphate pathway in the cellular metabolic network. This genetic modification successfully enabled the synthesis of sedoheptulose from glucose. Additionally, we identified key enzymes responsible for product formation through transcriptome analysis, and their corresponding genes were overexpressed, resulting in a further 20% increase in sedoheptulose production.
Conclusion: We achieved a sedoheptulose concentration of 24 g/L with a yield of 0.4 g/g glucose in a 1 L fermenter, marking the highest value up to date. The produced sedoheptulose could further function as feedstock for synthesising structural seven-carbon sugars through coupling with enzymatic isomerisation, epimerisation and reduction reactions.
(© 2024. The Author(s).)
References: J Biol Chem. 2015 Jul 24;290(30):18678-98. (PMID: 26071590)
J Mol Biol. 2006 Sep 1;361(5):1003-34. (PMID: 16889794)
J Org Chem. 2001 Jun 29;66(13):4625-9. (PMID: 11421783)
Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):E1974-83. (PMID: 25848029)
J Biochem. 1963 Jul;54:107-8. (PMID: 14056345)
Cancer Res. 1995 Aug 1;55(15):3278-85. (PMID: 7614462)
J Biotechnol. 2006 Aug 5;124(4):717-22. (PMID: 16716430)
Org Lett. 2020 Oct 16;22(20):8018-8022. (PMID: 32991182)
Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2818-2823. (PMID: 29483275)
Biotechnol Adv. 2017 Mar - Apr;35(2):267-274. (PMID: 28111316)
Biol Rev Camb Philos Soc. 2015 Aug;90(3):927-63. (PMID: 25243985)
Nat Commun. 2021 Jan 29;12(1):678. (PMID: 33514753)
Enzyme Microb Technol. 2018 Sep;116:16-22. (PMID: 29887012)
J Agric Food Chem. 2021 Jul 7;69(26):7367-7376. (PMID: 34170139)
J Biotechnol. 2003 Sep 4;104(1-3):287-99. (PMID: 12948646)
Metab Eng. 2017 Jul;42:19-24. (PMID: 28545807)
Biotechnol Biofuels. 2020 Oct 20;13:176. (PMID: 33093870)
Crit Rev Food Sci Nutr. 2018;58(16):2768-2778. (PMID: 28662355)
Chemistry. 2023 Nov 8;29(62):e202302277. (PMID: 37552007)
Biotechnol Bioeng. 2015 Jan;112(1):168-80. (PMID: 25060350)
Nat Prod Rep. 2021 Oct 20;38(10):1887-1909. (PMID: 33704304)
Appl Environ Microbiol. 2015 Jul;81(13):4284-94. (PMID: 25888171)
J Biol Chem. 2006 Nov 24;281(47):36149-61. (PMID: 16990279)
Biotechnol Biofuels Bioprod. 2023 Oct 29;16(1):162. (PMID: 37899467)
Metab Eng. 2022 Jan;69:134-146. (PMID: 34856366)
Nutrients. 2021 Dec 29;14(1):. (PMID: 35011030)
Appl Microbiol Biotechnol. 2021 May;105(10):4017-4031. (PMID: 33950280)
Biotechnol Bioeng. 2019 Apr;116(4):745-756. (PMID: 30597517)
معلومات مُعتمدة: TSBICIP-KJGG-008-03 and TSBICIP-PTJJ-007-10 Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project; TSBICIP-KJGG-008-03 and TSBICIP-PTJJ-007-10 Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project; TSBICIP-KJGG-008-03 and TSBICIP-PTJJ-007-10 Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project; TSBICIP-KJGG-008-03 and TSBICIP-PTJJ-007-10 Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project; No. 32271545 China National Key Research and Development; No. 32271545 China National Key Research and Development; No. 32271545 China National Key Research and Development; No. 32271545 Natural Science Foundation of China; No. 32271545 Natural Science Foundation of China; No. 32271545 Natural Science Foundation of China; 2021176 Youth Promotion Association of Chinese Academy of Sciences; 2021176 Youth Promotion Association of Chinese Academy of Sciences; 2021176 Youth Promotion Association of Chinese Academy of Sciences; JZ1-ZG2-2301-026 TIB Competitive Research Project; JZ1-ZG2-2301-026 TIB Competitive Research Project; JZ1-ZG2-2301-026 TIB Competitive Research Project
فهرسة مساهمة: Keywords: Corynebacterium glutamicum; Metabolic engineering; Sedoheptulose; Seven-carbon sugars
المشرفين على المادة: 0 (Heptoses)
IY9XDZ35W2 (Glucose)
3019-74-7 (sedoheptulose)
تواريخ الأحداث: Date Created: 20240913 Date Completed: 20240914 Latest Revision: 20240916
رمز التحديث: 20240916
مُعرف محوري في PubMed: PMC11401394
DOI: 10.1186/s12934-024-02501-2
PMID: 39272184
قاعدة البيانات: MEDLINE
الوصف
تدمد:1475-2859
DOI:10.1186/s12934-024-02501-2