دورية أكاديمية
أعرض في EDS

A nanobody-guided multifunctional T cell engager promotes strong anti-tumor responses via synergistic immuno-photothermal effects.

التفاصيل البيبلوغرافية
العنوان: A nanobody-guided multifunctional T cell engager promotes strong anti-tumor responses via synergistic immuno-photothermal effects.
المؤلفون: Xie S; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China.; Pharmaceutical College, Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Shi W; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Duan S; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China.; Department of Medicine, Guangxi University of Science and Technology, Liuzhou, Guangxi, 545005, P. R. China., Huang X; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Liu A; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Hou X; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China.; School of Basic Medical Science, Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Lin X; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Zhong D; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Sun S; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Ding Z; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China., Yang X; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China. yangxiaomei6009@sina.com.; School of Basic Medical Science, Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China. yangxiaomei6009@sina.com., Chen X; Nanomedicine Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore. chen.shawn@nus.edu.sg., Lu X; Guangxi Key Laboratory of Nanobody Research, Guangxi Nanobody Engineering Research Center, College of Stomatology, Hospital of Stomatology Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China. luxiaoling@gxmu.edu.cn.
المصدر: Journal of nanobiotechnology [J Nanobiotechnology] 2024 Sep 14; Vol. 22 (1), pp. 561. Date of Electronic Publication: 2024 Sep 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Single-Domain Antibodies*/chemistry , Single-Domain Antibodies*/immunology , T-Lymphocytes*/immunology , Indocyanine Green*/chemistry , Immunotherapy*/methods, Animals ; Mice ; Cell Line, Tumor ; Humans ; Neoplasms/therapy ; Neoplasms/immunology ; Female ; Mice, Inbred BALB C ; Photothermal Therapy/methods ; Liposomes/chemistry ; Lymphocyte Activation ; Mice, Inbred C57BL ; CD3 Complex/immunology
مستخلص: Background: T cell-based immunotherapies are facing great challenges in the recruitment and activation of tumor-specific T cells against solid tumors. Among which, utilizing nanobody (Nb) or nanobodies (Nbs) to construct T cell engager has emerged as a more practical potential for enhancing the anti-tumor effectiveness of T cells. Here, we designed a new Nb-guided multifunctional T cell engager (Nb-MuTE) that not only recruited effector T cells into the tumor tissues, but also efficiently activated T cells anti-tumor immunity when synergies with photothermal effect.
Results: The Nb-MuTE, which was constructed based on an indocyanine green (ICG)-containing liposome with surface conjugation of CD105 and CD3 Nbs, and showed excellent targetability to both tumor and T cells, following enhancement of activation, proliferation and cytokine secretion of tumor-specific T cells. Notably, the immunological anti-tumor functions of Nb-MuTE-mediated T cells were further enhanced by the ICG-induced photothermal effect in vitro and in vivo.
Conclusions: Such a new platform Nb-MuTE provides a practical and "all-in-one" strategy to potentiate T cell responses for the treatment of solid tumor in clinic.
(© 2024. The Author(s).)
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معلومات مُعتمدة: No.2023GXNSFBA026178 Guangxi Natural Science Foundation Youth Fund Project of China; NUHSRO/2021/034/TRP/09/Nanomedicine NUS School of Medicine Nanomedicine Translational Research Programme; No. 82220108003 the International (Regional) Cooperation and Exchange Program of National Natural Science Foundation of China
فهرسة مساهمة: Keywords: Nanobody; Photothermal therapy; Solid tumor immunotherapy; T cell; T cell engager
المشرفين على المادة: 0 (Single-Domain Antibodies)
IX6J1063HV (Indocyanine Green)
0 (Liposomes)
0 (CD3 Complex)
تواريخ الأحداث: Date Created: 20240913 Date Completed: 20240914 Latest Revision: 20240916
رمز التحديث: 20240916
مُعرف محوري في PubMed: PMC11401270
DOI: 10.1186/s12951-024-02781-1
PMID: 39272205
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-3155
DOI:10.1186/s12951-024-02781-1