دورية أكاديمية
أعرض في EDS

Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability.

التفاصيل البيبلوغرافية
العنوان: Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability.
المؤلفون: Sheng H; Department of Chemical and Biological Engineering, University of Idaho, Moscow, ID 83844, USA., Ndeddy Aka RJ; Department of Chemical and Biological Engineering, University of Idaho, Moscow, ID 83844, USA., Wu S; Department of Chemical and Biological Engineering, University of Idaho, Moscow, ID 83844, USA.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Aug 25; Vol. 25 (17). Date of Electronic Publication: 2024 Aug 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Flagella*/metabolism , Flagella*/genetics , Lipopolysaccharides*/biosynthesis , Escherichia coli O157*/genetics , Escherichia coli O157*/metabolism , Escherichia coli O157*/physiology , Biofilms*/growth & development, Animals ; Cattle ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; Escherichia coli Proteins/metabolism ; Escherichia coli Proteins/genetics ; Bacterial Adhesion ; Epithelial Cells/microbiology ; Epithelial Cells/metabolism
مستخلص: Escherichia coli O157:H7 ( E. coli O157) is known for causing severe foodborne illnesses such as hemorrhagic colitis and hemolytic uremic syndrome. Although E. coli O157 is typically regarded as an extracellular pathogen and a weak biofilm producer, some E. coli O157 strains, including a clinical strain ATCC 43895, exhibit a notable ability to invade bovine crypt cells and other epithelial cells, as well as to form robust biofilm. This invasive strain persists in the bovine host significantly longer than non-invasive strains. Various surface-associated factors, including lipopolysaccharides (LPS), flagella, and other adhesins, likely contribute to this enhanced invasiveness and biofilm formation. In this study, we constructed a series of LPS-core deletion mutations ( waaI , waaG , waaF , and waaC ) in E. coli O157 ATCC 43895, resulting in stepwise truncations of the LPS. This approach enabled us to investigate the effects on the biosynthesis of key surface factors, such as flagella and curli, and the ability of this invasive strain to invade host cells. We confirmed the LPS structure and found that all LPS-core mutants failed to form biofilms, highlighting the crucial role of core oligosaccharides in biofilm formation. Additionally, the LPS inner-core mutants Δ waaF and Δ waaC lost the ability to produce flagella and curli. Furthermore, these inner-core mutants exhibited a dramatic reduction in adherence to and invasion of epithelial cells (MAC-T), showing an approximately 100-fold decrease in cell invasion compared with the outer-core mutants ( waaI and waaG ) and the wild type. These findings underscore the critical role of LPS-core truncation in impairing flagella and curli biosynthesis, thereby reducing the invasion capability of E. coli O157 ATCC 43895.
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فهرسة مساهمة: Keywords: LPS core; bovine host; cell invasion; invasive E. coli O157:H7; production of flagella and curli
المشرفين على المادة: 0 (Lipopolysaccharides)
148349-72-8 (Crl protein, Bacteria)
0 (Bacterial Proteins)
0 (Escherichia coli Proteins)
تواريخ الأحداث: Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240916
رمز التحديث: 20240916
مُعرف محوري في PubMed: PMC11394844
DOI: 10.3390/ijms25179224
PMID: 39273173
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25179224