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FABP5 Is a Possible Factor for the Maintenance of Functions of Human Non-Pigmented Ciliary Epithelium Cells.

التفاصيل البيبلوغرافية
العنوان: FABP5 Is a Possible Factor for the Maintenance of Functions of Human Non-Pigmented Ciliary Epithelium Cells.
المؤلفون: Higashide M; Departments of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan., Watanabe M; Departments of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan., Sato T; Department of Cardiovascular, Renal and Metabolic Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.; Department of Cellular Physiology and Signal Transduction, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan., Umetsu A; Departments of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan., Nishikiori N; Departments of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan., Ogawa T; Department of Cardiovascular, Renal and Metabolic Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.; Department of Cellular Physiology and Signal Transduction, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan., Furuhashi M; Department of Cardiovascular, Renal and Metabolic Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan., Ohguro H; Departments of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Aug 27; Vol. 25 (17). Date of Electronic Publication: 2024 Aug 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Fatty Acid-Binding Proteins*/metabolism , Fatty Acid-Binding Proteins*/genetics, Humans ; Cell Line ; Retinal Pigment Epithelium/metabolism ; Retinal Pigment Epithelium/cytology ; Epithelial Cells/metabolism ; Mitochondria/metabolism ; Mitochondria/genetics ; Gene Expression Regulation ; Ciliary Body/metabolism ; Ciliary Body/cytology ; Glycolysis
مستخلص: To elucidate the possible biological roles of fatty acid-binding protein 5 (FABP5) in the intraocular environment, the cells from which FABP5 originates were determined by using four different intraocular tissue-derived cell types including human non-pigmented ciliary epithelium (HNPCE) cells, retinoblastoma (RB) cells, adult retinal pigment epithelial19 (ARPE19) cells and human ocular choroidal fibroblast (HOCF) cell lines, and the effects of FABP ligand 6, a specific inhibitor for FABP5 and FABP7 were analyzed by RNA sequencing and seahorse cellular metabolic measurements. Among these four different cell types, qPCR analysis showed that FABP5 was most prominently expressed in HNPCE cells, in which no mRNA expression of FABP7 was detected. In RNA sequencing analysis, 166 markedly up-regulated and 198 markedly down-regulated differentially expressed genes (DEGs) were detected between non-treated cells and cells treated with FABP ligand 6. IPA analysis of these DEGs suggested that FABP5 may be involved in essential roles required for cell development, cell survival and cell homeostasis. In support of this possibility, both mitochondrial and glycolytic functions of HNPCE cells, in which mRNA expression of FABP5, but not that of FABP7, was detected, were shown by using a Seahorse XFe96 Bioanalyzer to be dramatically suppressed by FABP ligand 6-induced inhibition of the activity of FABP5. Furthermore, in IPA upstream analysis, various unfolded protein response (UPR)-related factors were identified as upstream and causal network master regulators. Analysis by qPCR analysis showed significant upregulation of the mRNA expression of most of UPR-related factors and aquaporin1 (AQP1). The findings in this study suggest that HNPCE is one of intraocular cells producing FABP5 and may be involved in the maintenance of UPR and AQP1-related functions of HNPCE.
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فهرسة مساهمة: Keywords: FABP5; RNA sequencing; glycolysis; human non-pigmented ciliary epithelium cells (HNPCE); ingenuity pathway analysis (IPA); mitochondrial respiration; seahorse bioanalyzer
المشرفين على المادة: 0 (FABP5 protein, human)
0 (Fatty Acid-Binding Proteins)
تواريخ الأحداث: Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240916
رمز التحديث: 20240916
مُعرف محوري في PubMed: PMC11394871
DOI: 10.3390/ijms25179285
PMID: 39273233
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25179285