دورية أكاديمية
أعرض في EDS

Raman Spectroscopy of Optically Trapped Living Human T Cell Subsets and Monocytes.

التفاصيل البيبلوغرافية
العنوان: Raman Spectroscopy of Optically Trapped Living Human T Cell Subsets and Monocytes.
المؤلفون: Nötzel M; Department of Neurology, Technische Universität Dresden, 01307 Dresden, Germany., Mahamid M; Department of Neurology, Technische Universität Dresden, 01307 Dresden, Germany., Kronstein-Wiedemann R; Department of Transfusion Medicine, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Ziemssen T; Department of Neurology, Technische Universität Dresden, 01307 Dresden, Germany., Akgün K; Department of Neurology, Technische Universität Dresden, 01307 Dresden, Germany.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Sep 03; Vol. 25 (17). Date of Electronic Publication: 2024 Sep 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Spectrum Analysis, Raman*/methods , Monocytes*/cytology , Monocytes*/immunology , T-Lymphocyte Subsets*/immunology , T-Lymphocyte Subsets*/metabolism, Humans ; Optical Tweezers ; CD8-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/immunology ; Lymphocyte Activation ; CD28 Antigens/metabolism ; CD28 Antigens/immunology
مستخلص: In recent years, Raman spectroscopy has garnered growing interest in the field of biomedical research. It offers a non-invasive and label-free approach to defining the molecular fingerprint of immune cells. We utilized Raman spectroscopy on optically trapped immune cells to investigate their molecular compositions. While numerous immune cell types have been studied in the past, the characterization of living human CD3/CD28-stimulated T cell subsets remains incomplete. In this study, we demonstrate the capability of Raman spectroscopy to readily distinguish between naïve and stimulated CD4 and CD8 cells. Additionally, we compared these cells with monocytes and discovered remarkable similarities between stimulated T cells and monocytes. This paper contributes to expanding our knowledge of Raman spectroscopy of immune cells and serves as a launching point for future clinical applications.
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فهرسة مساهمة: Keywords: CD3/CD28 stimulation; CD4; CD8; Raman spectroscopy; T cells; monocytes; optical trapping
المشرفين على المادة: 0 (CD28 Antigens)
تواريخ الأحداث: Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240916
رمز التحديث: 20240916
مُعرف محوري في PubMed: PMC11394728
DOI: 10.3390/ijms25179557
PMID: 39273504
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25179557