دورية أكاديمية
Evaluation of the effect of clonazepam and its metabolites on the life cycle of Megaselia scalaris (Loew) (Diptera: Phoridae).
| العنوان: | Evaluation of the effect of clonazepam and its metabolites on the life cycle of Megaselia scalaris (Loew) (Diptera: Phoridae). |
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| المؤلفون: | Quijano-Mateos A; National Autonomous University of Mexico, National School of Forensic Sciences, Mexico City, Mexico., Castillo-Alanis A; National Autonomous University of Mexico, National School of Forensic Sciences, Mexico City, Mexico., Pedraza-Lara CS; National Autonomous University of Mexico, National School of Forensic Sciences, Mexico City, Mexico., Bravo-Gómez ME; National Autonomous University of Mexico, National School of Forensic Sciences, Mexico City, Mexico. Electronic address: mebravo@unam.mx. |
| المصدر: | Science & justice : journal of the Forensic Science Society [Sci Justice] 2024 Sep; Vol. 64 (5), pp. 460-465. Date of Electronic Publication: 2024 Jul 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 9508563 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4452 (Electronic) Linking ISSN: 13550306 NLM ISO Abbreviation: Sci Justice Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Elsevier Original Publication: Harrogate, North Yorkshire, UK ; Middlesex, NJ : The Society, c1995- |
| مواضيع طبية MeSH: | Clonazepam*/pharmacology , Larva*/growth & development , Larva*/drug effects , Diptera*/growth & development , Diptera*/drug effects , Diptera*/metabolism , Forensic Entomology*, Animals ; Postmortem Changes ; Life Cycle Stages/drug effects |
| مستخلص: | Fly colonization patterns and development are crucial in estimating the post-mortem interval (PMI) of decomposing corpses. Understanding the potential effects of xenobiotics on species development in cadaveric entomofauna is essential for accurate PMI estimation, given their presence in decomposing bodies. Benzodiazepines, commonly prescribed for their anxiolytic, hypnotic, and muscle relaxant effects, are of forensic interest due to their potential for abuse, dependence, intoxication, and overdose-related deaths. This study aimed to explore the effect of clonazepam and its metabolites on Megaselia scalaris, a species commonly used to estimate PMI, the alteration of which could impact the accuracy of said estimation. The S9 biotransformation fraction, an in vitro model consisting of an array of metabolic enzymes, was used to generate phase I and II metabolites for evaluating their effect on M. scalaris development, representing an innovative approach to this type of study. Megaselia scalaris larvae were reared in synthetic growth media under controlled conditions. The study compared different groups: control, clonazepam, and clonazepam with S9 fraction. Larvae were measured daily to determine growth rate, and clonazepam concentrations were analyzed using HPLC-DAD. Results showed that larvae grown in media containing clonazepam or clonazepam with S9 fraction developed faster than control larvae, reaching their pupal stage earlier. Growth rates were also altered in treated groups. In conclusion, the presence of clonazepam and its metabolites accelerated the life cycle of M. scalaris, potentially impacting the accuracy of PMI estimation. These findings underscore the importance of considering xenobiotics in forensic entomological studies for precise post-mortem interval determination. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Clonazepam; Entomotoxicology; Megaselia scalaris; Metabolites; PMI |
| المشرفين على المادة: | 5PE9FDE8GB (Clonazepam) |
| تواريخ الأحداث: | Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240914 |
| رمز التحديث: | 20240916 |
| DOI: | 10.1016/j.scijus.2024.07.002 |
| PMID: | 39277328 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1876-4452 |
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| DOI: | 10.1016/j.scijus.2024.07.002 |