دورية أكاديمية
أعرض في EDS

The potential of human leukocyte antigen alleles to assist with multiple-contributor DNA mixtures: Proof of concept study.

التفاصيل البيبلوغرافية
العنوان: The potential of human leukocyte antigen alleles to assist with multiple-contributor DNA mixtures: Proof of concept study.
المؤلفون: Kuffel A; Leverhulme Research Centre for Forensic Science, Fleming Gym Building, University of Dundee, Small's Wynd, Dundee, United Kingdom. Electronic address: akuffel001@dundee.ac.uk., Cole C; Health Informatics Centre, Division of Population and Health Genomics, School of Medicine, University of Dundee, Dundee, United Kingdom. Electronic address: c.cole@dundee.ac.uk., Mallinder B; SPA Forensic Services, Rushton Court, 3 West Victoria Dock Road, Dundee, United Kingdom. Electronic address: ben.mallinder@spa.police.uk., Nic Daeid N; Leverhulme Research Centre for Forensic Science, Fleming Gym Building, University of Dundee, Small's Wynd, Dundee, United Kingdom. Electronic address: n.nicdaeid@dundee.ac.uk., Gray A; Leverhulme Research Centre for Forensic Science, Fleming Gym Building, University of Dundee, Small's Wynd, Dundee, United Kingdom. Electronic address: a.z.gray@dundee.ac.uk.
المصدر: Science & justice : journal of the Forensic Science Society [Sci Justice] 2024 Sep; Vol. 64 (5), pp. 533-542. Date of Electronic Publication: 2024 Aug 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 9508563 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4452 (Electronic) Linking ISSN: 13550306 NLM ISO Abbreviation: Sci Justice Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Elsevier
Original Publication: Harrogate, North Yorkshire, UK ; Middlesex, NJ : The Society, c1995-
مواضيع طبية MeSH: Alleles* , HLA Antigens*/genetics , DNA Fingerprinting*/methods , DNA*/genetics , Proof of Concept Study*, Humans ; Genetic Markers ; Microsatellite Repeats
مستخلص: One of the most challenging issues still present in forensic DNA analysis is identifying individuals in samples containing DNA from multiple contributors. The introduction of novel identification markers may be a useful tool in the deconvolution of such DNA mixtures. In this study, we investigated the potential of alleles from the human leukocyte antigen system (HLA) to aid in identifying individuals in complex, multiple-donor DNA samples. The most advantageous characteristic of the HLA complex is its polymorphism in the human genome. A 22-loci multiplex with HLA markers was designed and applied to two-, three-, and four-person DNA mixtures. The results of the conducted experiments demonstrated that the identification of individuals in multiple contributor samples with the help of HLA markers is possible; however, it is clear that the reliability of the method is heavily dependent on the number of unique alleles for each individual in the analysed mixture. In order to compare this novel approach against the already established process, the same group of reference and multiple-contributor samples was analysed with a commonly used set of STR markers. This proof-of-concept research shows the importance of examining alternative solutions to the current deconvolution challenge in forensic DNA profiling.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: DNA analysis; DNA mixtures; Human leukocyte antigens; Massively parallel sequencing; Novel forensic DNA markers
المشرفين على المادة: 0 (HLA Antigens)
9007-49-2 (DNA)
0 (Genetic Markers)
تواريخ الأحداث: Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240914
رمز التحديث: 20240916
DOI: 10.1016/j.scijus.2024.08.001
PMID: 39277335
قاعدة البيانات: MEDLINE
الوصف
تدمد:1876-4452
DOI:10.1016/j.scijus.2024.08.001