دورية أكاديمية
أعرض في EDS

Water-soluble garlic polysaccharide (WSGP) improves ulcerative volitis by modulating the intestinal barrier and intestinal flora metabolites.

التفاصيل البيبلوغرافية
العنوان: Water-soluble garlic polysaccharide (WSGP) improves ulcerative volitis by modulating the intestinal barrier and intestinal flora metabolites.
المؤلفون: Shao X; Department of Critical Care Medicine, Maoming People's Hospital, Maoming, 525000, Guangdong, China.; Department of Food Science and Engineering, Jinan University, Guangzhou, 510632, Guangdong, China., Li J; Department of Food Science and Engineering, Jinan University, Guangzhou, 510632, Guangdong, China., Shao Q; Department of Cell Biology, Jinan University, Guangzhou, 510632, Guangdong, China., Qu R; Department of Intensive Care Unit of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, Guangdong, China., Ouyang X; Department of Intensive Care Unit of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, Guangdong, China., Wang Y; Department of Food Science and Engineering, Jinan University, Guangzhou, 510632, Guangdong, China. twyong@jnu.edu.cn., Chen C; Department of Critical Care Medicine, Maoming People's Hospital, Maoming, 525000, Guangdong, China. gghccm@163.com.; Department of Critical Care Medicine, Shenzhen People's Hospital, Shenzhen, 518001, Guangdong, China. gghccm@163.com.
المصدر: Scientific reports [Sci Rep] 2024 Sep 14; Vol. 14 (1), pp. 21504. Date of Electronic Publication: 2024 Sep 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Gastrointestinal Microbiome*/drug effects , Intestinal Mucosa*/metabolism , Intestinal Mucosa*/drug effects , Intestinal Mucosa*/microbiology , Intestinal Mucosa*/pathology , Polysaccharides*/pharmacology , Garlic*/chemistry , Colitis, Ulcerative*/microbiology , Colitis, Ulcerative*/drug therapy , Colitis, Ulcerative*/metabolism , Colitis, Ulcerative*/pathology, Animals ; Mice ; Disease Models, Animal ; Male ; Colon/metabolism ; Colon/pathology ; Colon/drug effects ; Colon/microbiology ; Signal Transduction/drug effects ; NF-kappa B/metabolism ; STAT3 Transcription Factor/metabolism ; Water ; Mice, Inbred C57BL
مستخلص: WSGP has demonstrated significant potential for various bioactive effects. However, limited research has explored their anti-ulcerative colitis (UC) effects and mechanism on the colonic system and gut microbial metabolites. We evaluated the ameliorative effects of WSGP on the UC mice model. Using H&E to assess histological injury of colon morphology, AB-PAS staining to detect mucin secretion from goblet cells and the mucous layer, IF to evaluate the expression of intercellular tight junction proteins, ELISA to measure inflammatory factors, WB analysis to measure protein expression of inflammatory signaling pathways, RT-qPCR to quantify gene transcription of inflammatory factors, and LC-MS to analyze metabolites in mouse cecum contents. WSGP supplementation increased food intake, body weight, and colon length while reducing disease activity and histological scores in colitis-afflicted mice. WSGP mitigated colonic tissue damage and restored intestinal barrier integrity by suppressing NF-κB/STAT3 signaling, thereby decreasing gene transcription, protein expression of proinflammatory factors, and nitric oxide production. Additionally, WSGP improved UC by altering the variety of intestinal microbial metabolites. This study demonstrates that WSGP supplementation attenuates UC mice by suppressing the NF-κB/STAT3 signaling pathway, enhancing mucosal barrier function, reducing pro-inflammatory cytokines, and modulating gut microbial metabolites.
(© 2024. The Author(s).)
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معلومات مُعتمدة: B2023048 Guangdong Medical Research Fund Project; 200221115835503 Office of Talent Work Leading Team in Maoming (MaoRenCaiBan (2020) 24, Project)
فهرسة مساهمة: Keywords: Flora metabolites; Garlic polysaccharide; Inflammation; NF-κB/STAT3; Ulcerative colitis
المشرفين على المادة: 0 (Polysaccharides)
0 (NF-kappa B)
0 (STAT3 Transcription Factor)
059QF0KO0R (Water)
تواريخ الأحداث: Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20240917
رمز التحديث: 20240917
مُعرف محوري في PubMed: PMC11401863
DOI: 10.1038/s41598-024-72797-y
PMID: 39277703
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-72797-y