دورية أكاديمية
Isolation and Cryopreservation of Primary Macaque Hepatocytes.
| العنوان: | Isolation and Cryopreservation of Primary Macaque Hepatocytes. |
|---|---|
| المؤلفون: | Guo X; Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, Arkansas., Seo JE; Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, Arkansas., Davis K; Toxicologic Pathology Associates, Jefferson, Arkansas., Malhi P; Toxicologic Pathology Associates, Jefferson, Arkansas., Fili C; Veterinary Services, Office of Scientific Coordination, National Center for Toxicological Research, Jefferson, Arkansas. |
| المصدر: | Current protocols [Curr Protoc] 2024 Sep; Vol. 4 (9), pp. e70015. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101773894 Publication Model: Print Cited Medium: Internet ISSN: 2691-1299 (Electronic) Linking ISSN: 26911299 NLM ISO Abbreviation: Curr Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hoboken, NJ : John Wiley & Sons, [2021]- |
| مواضيع طبية MeSH: | Hepatocytes*/cytology , Hepatocytes*/drug effects , Cryopreservation*/methods , Macaca mulatta*, Animals ; Cell Separation/methods ; Liver/cytology ; Perfusion/methods ; Cells, Cultured |
| مستخلص: | Primary human hepatocytes (PHHs) are recognized as the "gold standard" for evaluating toxicity of various drugs or chemicals in vitro. However, due to their limited availability, primary hepatocytes isolated from rodents are more commonly used in various experimental studies than PHHs. However, bigger differences in drug metabolism were seen between humans and rats compared to those between human and non-human primates. Here, we describe a method to isolate primary hepatocytes from the liver of rhesus macaques (Macaca mulatta, a species of Old-World monkey) after in situ whole liver perfusion. Techniques for cryopreserving and recovering primary macaque hepatocytes (PMHs) are also described. Given the remarkable physiological and genetic similarity of non-human primates to humans, PMHs isolated using this protocol may serve as a reliable surrogate of PHHs in toxicological research and preclinical studies. Published 2024. This article is a U.S. Government work and is in the public domain in the USA. Basic Protocol 1: In situ whole liver perfusion Basic Protocol 2: Primary macaque hepatocyte isolation and cell plating Basic Protocol 3: Cryopreservation and recovery of primary macaque hepatocytes. (Published 2024. This article is a U.S. Government work and is in the public domain in the USA.) |
| References: | Berry, M. N., & Friend, D. S. (1969). High‐yield preparation of isolated rat liver parenchymal cells: A biochemical and fine structural study. Journal of Cell Biology, 43(3), 506–520. https://doi.org/10.1083/jcb.43.3.506. Chang, S. Y., Voellinger, J. L., van Ness, K. P., Chapron, B., Shaffer, R. M., Neumann, T., White, C. C., Kavanagh, T. J., Kelly, E. J., & Eaton, D. L. (2017). Characterization of rat or human hepatocytes cultured in microphysiological systems (MPS) to identify hepatotoxicity. Toxicology in Vitro, 40, 170–183. https://doi.org/10.1016/j.tiv.2017.01.007. Charni‐Natan, M., & Goldstein, I. (2020). Protocol for primary mouse hepatocyte isolation. STAR Protocols, 1(2), 100086. https://doi.org/10.1016/j.xpro.2020.100086. Elvevold, K., Kyrrestad, I., & Smedsrød, B. (2022). Protocol for isolation and culture of mouse hepatocytes (HCs), Kupffer cells (KCs), and liver sinusoidal endothelial cells (LSECs) in analyses of hepatic drug distribution. Methods in Molecular Biology, 2434, 385–402. https://doi.org/10.1007/978‐1‐0716‐2010‐6_27. Grow, D. A., McCarrey, J. R., & Navara, C. S. (2016). Advantages of nonhuman primates as preclinical models for evaluating stem cell‐based therapies for Parkinson's disease. Stem Cell Research, 17(2), 352–366. https://doi.org/10.1016/j.scr.2016.08.013. Harding, J. D. (2017). Nonhuman primates and translational research: Progress, opportunities, and challenges. ILAR Journal, 58(2), 141–150. https://doi.org/10.1093/ilar/ilx033. Jain, N. K., & Roy, I. (2010). Trehalose and protein stability. Current Protocols in Protein Science, 59, 4.9.1–4.9.12. https://doi.org/10.1002/0471140864.ps0409s59. Johnston, D. E., & Jasuja, R. (1994). Purification of cultured primary rat hepatocytes using selection with ricin A subunit. Hepatology, 20(2), 436–444. LeCluyse, E. L., Alexandre, E., Hamilton, G. A., Viollon‐Abadie, C., Coon, D. J., Jolley, S., & Richert, L. (2005). Isolation and culture of primary human hepatocytes. Methods in Molecular Biology, 290, 207–229. https://doi.org/10.1385/1‐59259‐838‐2:207. MacArthur Clark, J. (2018). The 3Rs in research: A contemporary approach to replacement, reduction and refinement. British Journal of Nutrition, 120(s1), S1–S7. https://doi.org/10.1017/s0007114517002227. Martinetti, D., Colarossi, C., Buccheri, S., Denti, G., Memeo, L., & Vicari, L. (2017). Effect of trehalose on cryopreservation of pure peripheral blood stem cells. Biomedical Reports, 6(3), 314–318. https://doi.org/10.3892/br.2017.859. McNulty, W. P. (1985). Toxicity and fetotoxicity of TCDD, TCDF and PCB isomers in rhesus macaques (Macaca mulatta). Environmental Health Perspectives, 60, 77–88. https://doi.org/10.1289/ehp.856077. Monteith, D. K., & Theiss, J. C. (1996). Comparison of tacrine‐induced cytotoxicity in primary cultures of rat, mouse, monkey, dog, rabbit, and human hepatocytes. Drug and Chemical Toxicology, 19(1‐2), 59–70. https://doi.org/10.3109/01480549609002196. Mudry, M. D., Martinez, R. A., Nieves, M., & Carballo, M. A. (2011). Biomarkers of genotoxicity and genomic instability in a non‐human primate, Cebus libidinosus (Cebidae, Platyrrhini), exposed to nitroimidazole derivatives. Mutation Research, 721(1), 108–113. https://doi.org/10.1016/j.mrgentox.2011.01.002. Ng, I. C., Zhang, L., Shen, N. N. Y. Y., Soong, Y. T., Ng, C. W., Koh, P. K. S., Zhou, Y., & Yu, H. (2021). Isolation of primary rat hepatocytes with multiparameter perfusion control. Journal of Visualized Experiments, 170, e62289. https://doi.org/10.3791/62289. Price, R. J., Mistry, H., Wield, P. T., Renwick, A. B., Beamand, J. A., & Lake, B. G. (1996). Comparison of the toxicity of allyl alcohol, coumarin and menadione in precision‐cut rat, guinea‐pig, Cynomolgus monkey and human liver slices. Archives of Toxicology, 71(1‐2), 107–111. https://doi.org/10.1007/s002040050364. Seo, J. E., Davis, K., Malhi, P., He, X., Bryant, M., Talpos, J., Burks, S., Mei, N., & Guo, X. (2021). Genotoxicity evaluation using primary hepatocytes isolated from rhesus macaque (Macaca mulatta). Toxicology, 462, 152936. https://doi.org/10.1016/j.tox.2021.152936. Shen, L., Hillebrand, A., Wang, D. Q., & Liu, M. (2012). Isolation and primary culture of rat hepatic cells. Journal of Visualized Experiments, 64, e3917. https://doi.org/10.3791/3917. Shulman, M., & Nahmias, Y. (2013). Long‐term culture and coculture of primary rat and human hepatocytes. Methods in Molecular Biology, 945, 287–302. https://doi.org/10.1007/978‐1‐62703‐125‐7_17. Smolarek, T. A., Higgins, C. V., & Amacher, D. E. (1990). Metabolism and cytotoxicity of acetaminophen in hepatocyte cultures from rat, rabbit, dog, and monkey. Drug Metabolism and Disposition, 18(5), 659–663. Steensma, A., Beamand, J. A., Walters, D. G., Price, R. J., & Lake, B. G. (1994). Metabolism of coumarin and 7‐ethoxycoumarin by rat, mouse, guinea pig, cynomolgus monkey and human precision‐cut liver slices. Xenobiotica, 24(9), 893–907. https://doi.org/10.3109/00498259409043288. Sun, E. L., Aspar, D. G., Ulrich, R. G., & Melchior, G. W. (1990). Cryopreservation of cynomolgus monkey (Macaca fascicularis) hepatocytes for subsequent culture and protein synthesis studies. In Vitro Cellular & Developmental Biology, 26(2), 147–150. https://doi.org/10.1007/bf02624105. Ulrich, R. G., Aspar, D. G., Cramer, C. T., Kletzien, R. F., & Ginsberg, L. C. (1990). Isolation and culture of hepatocytes from the cynomolgus monkey (Macaca fascicularis). In Vitro Cellular & Developmental Biology, 26(8), 815–823. https://www.ncbi.nlm.nih.gov/pubmed/1975577. Vorrink, S. U., Zhou, Y., Ingelman‐Sundberg, M., & Lauschke, V. M. (2018). Prediction of drug‐induced hepatotoxicity using long‐term stable primary hepatic 3D spheroid cultures in chemically defined conditions. Toxicological Sciences, 163(2), 655–665. https://doi.org/10.1093/toxsci/kfy058. |
| فهرسة مساهمة: | Keywords: cryopreservation; hepatocyte isolation; liver perfusion; primary macaque hepatocytes |
| تواريخ الأحداث: | Date Created: 20240916 Date Completed: 20240916 Latest Revision: 20240916 |
| رمز التحديث: | 20240917 |
| DOI: | 10.1002/cpz1.70015 |
| PMID: | 39283005 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2691-1299 |
|---|---|
| DOI: | 10.1002/cpz1.70015 |